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Transcriptome Analysis of Bacillus amyloliquefaciens Reveals Fructose Addition Effects on Fengycin Synthesis

Fengycin is a lipopeptide produced by Bacillus that has a strong inhibitory effect on filamentous fungi; however, its use is restricted due to poor production and low yield. Previous studies have shown that fengycin biosynthesis in B. amyloliquefaciens was found to be significantly increased after f...

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Autores principales: Lu, Hedong, Xu, Hai, Yang, Panping, Bilal, Muhammad, Zhu, Shaohui, Zhong, Mengyuan, Zhao, Li, Gu, Chengyuan, Liu, Shuai, Zhao, Yuping, Geng, Chengxin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9222730/
https://www.ncbi.nlm.nih.gov/pubmed/35741746
http://dx.doi.org/10.3390/genes13060984
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author Lu, Hedong
Xu, Hai
Yang, Panping
Bilal, Muhammad
Zhu, Shaohui
Zhong, Mengyuan
Zhao, Li
Gu, Chengyuan
Liu, Shuai
Zhao, Yuping
Geng, Chengxin
author_facet Lu, Hedong
Xu, Hai
Yang, Panping
Bilal, Muhammad
Zhu, Shaohui
Zhong, Mengyuan
Zhao, Li
Gu, Chengyuan
Liu, Shuai
Zhao, Yuping
Geng, Chengxin
author_sort Lu, Hedong
collection PubMed
description Fengycin is a lipopeptide produced by Bacillus that has a strong inhibitory effect on filamentous fungi; however, its use is restricted due to poor production and low yield. Previous studies have shown that fengycin biosynthesis in B. amyloliquefaciens was found to be significantly increased after fructose addition. This study investigated the effect of fructose on fengycin production and its regulation mechanism in B. amyloliquefaciens by transcriptome sequencing. According to the RNA sequencing data, 458 genes were upregulated and 879 genes were downregulated. Transcriptome analysis results showed that fructose changed the transcription of amino acid synthesis, fatty acid metabolism, and energy metabolism; alterations in these metabolic pathways contribute to the synthesis of fengycin. In an MLF medium (modified Landy medium with fructose), the expression level of the fengycin operon was two-times higher than in an ML medium (modified Landy medium). After fructose was added to B. amyloliquefaciens, the fengycin-synthesis-associated genes were activated in the process of fengycin synthesis.
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spelling pubmed-92227302022-06-24 Transcriptome Analysis of Bacillus amyloliquefaciens Reveals Fructose Addition Effects on Fengycin Synthesis Lu, Hedong Xu, Hai Yang, Panping Bilal, Muhammad Zhu, Shaohui Zhong, Mengyuan Zhao, Li Gu, Chengyuan Liu, Shuai Zhao, Yuping Geng, Chengxin Genes (Basel) Article Fengycin is a lipopeptide produced by Bacillus that has a strong inhibitory effect on filamentous fungi; however, its use is restricted due to poor production and low yield. Previous studies have shown that fengycin biosynthesis in B. amyloliquefaciens was found to be significantly increased after fructose addition. This study investigated the effect of fructose on fengycin production and its regulation mechanism in B. amyloliquefaciens by transcriptome sequencing. According to the RNA sequencing data, 458 genes were upregulated and 879 genes were downregulated. Transcriptome analysis results showed that fructose changed the transcription of amino acid synthesis, fatty acid metabolism, and energy metabolism; alterations in these metabolic pathways contribute to the synthesis of fengycin. In an MLF medium (modified Landy medium with fructose), the expression level of the fengycin operon was two-times higher than in an ML medium (modified Landy medium). After fructose was added to B. amyloliquefaciens, the fengycin-synthesis-associated genes were activated in the process of fengycin synthesis. MDPI 2022-05-31 /pmc/articles/PMC9222730/ /pubmed/35741746 http://dx.doi.org/10.3390/genes13060984 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Lu, Hedong
Xu, Hai
Yang, Panping
Bilal, Muhammad
Zhu, Shaohui
Zhong, Mengyuan
Zhao, Li
Gu, Chengyuan
Liu, Shuai
Zhao, Yuping
Geng, Chengxin
Transcriptome Analysis of Bacillus amyloliquefaciens Reveals Fructose Addition Effects on Fengycin Synthesis
title Transcriptome Analysis of Bacillus amyloliquefaciens Reveals Fructose Addition Effects on Fengycin Synthesis
title_full Transcriptome Analysis of Bacillus amyloliquefaciens Reveals Fructose Addition Effects on Fengycin Synthesis
title_fullStr Transcriptome Analysis of Bacillus amyloliquefaciens Reveals Fructose Addition Effects on Fengycin Synthesis
title_full_unstemmed Transcriptome Analysis of Bacillus amyloliquefaciens Reveals Fructose Addition Effects on Fengycin Synthesis
title_short Transcriptome Analysis of Bacillus amyloliquefaciens Reveals Fructose Addition Effects on Fengycin Synthesis
title_sort transcriptome analysis of bacillus amyloliquefaciens reveals fructose addition effects on fengycin synthesis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9222730/
https://www.ncbi.nlm.nih.gov/pubmed/35741746
http://dx.doi.org/10.3390/genes13060984
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