Cargando…

Determination of Intra- and Extracellular Metabolic Adaptations of 3D Cell Cultures upon Challenges in Real-Time by NMR

NMR flow devices provide longitudinal real-time quantitative metabolome characterisation of living cells. However, discrimination of intra- and extracellular contributions to the spectra represents a major challenge in metabolomic NMR studies. The present NMR study demonstrates the possibility to qu...

Descripción completa

Detalles Bibliográficos
Autores principales:	Urzì, Christian, Hertig, Damian, Meyer, Christoph, Maddah, Sally, Nuoffer, Jean-Marc, Vermathen, Peter
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	MDPI 2022
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9223855/ https://www.ncbi.nlm.nih.gov/pubmed/35743000 http://dx.doi.org/10.3390/ijms23126555

Ejemplares similares

Live monitoring of cellular metabolism and mitochondrial respiration in 3D cell culture system using NMR spectroscopy
por: Hertig, Damian, et al.
Publicado: (2021)

Intracellular Fate of the Photosensitizer Chlorin e4 with Different Carriers and Induced Metabolic Changes Studied by (1)H NMR Spectroscopy
por: Vermathen, Martina, et al.
Publicado: (2023)

Combined transcriptome and metabolome analyses of metformin effects reveal novel links between metabolic networks in steroidogenic systems
por: Udhane, Sameer S., et al.
Publicado: (2017)

(1)H HR-MAS NMR Based Metabolic Profiling of Lung Cancer Cells with Induced and De-Induced Cisplatin Resistance to Reveal Metabolic Resistance Adaptations
por: Vermathen, Martina, et al.
Publicado: (2021)

Metabolomic Profiling of Wildtype and Transgenic Giardia lamblia Strains by (1)H HR-MAS NMR Spectroscopy
por: Müller, Joachim, et al.
Publicado: (2020)

Probing the Interactions of Porphyrins with Macromolecules Using NMR Spectroscopy Techniques
por: Gjuroski, Ilche, et al.
Publicado: (2021)

Targeting lactate dehydrogenase B-dependent mitochondrial metabolism affects tumor initiating cells and inhibits tumorigenesis of non-small cell lung cancer by inducing mtDNA damage
por: Deng, Haibin, et al.
Publicado: (2022)

(1)H HR-MAS NMR Based Metabolic Profiling of Cells in Response to Treatment with a Hexacationic Ruthenium Metallaprism as Potential Anticancer Drug
por: Vermathen, Martina, et al.
Publicado: (2015)

Sampling Method Affects HR-MAS NMR Spectra of Healthy Caprine Brain Biopsies
por: Häni, Annakatrin, et al.
Publicado: (2021)

NMR quantification of diffusional exchange in cell suspensions with relaxation rate differences between intra and extracellular compartments
por: Eriksson, Stefanie, et al.
Publicado: (2017)

Extracellular Vesicles from Plants: Current Knowledge and Open Questions
por: Urzì, Ornella, et al.
Publicado: (2021)

(1)H HR-MAS NMR-Based Metabolomics of Cancer Cells in Response to Treatment with the Diruthenium Trithiolato Complex [(p-MeC(6)H(4)(i)Pr)(2)Ru(2)(SC(6)H(4)-p-Bu(t))(3)](+) (DiRu-1)
por: Primasová, Hedvika, et al.
Publicado: (2019)

The dark side of foetal bovine serum in extracellular vesicle studies
por: Urzì, Ornella, et al.
Publicado: (2022)

The Effect of Variations in the Intra- and Extracellular Ion Concentrations upon the Electrical Activity of Normal and Dystrophic Mouse Skeletal Muscle
por: Mclennan, H.
Publicado: (1961)

Comparing PVP and Polymeric Micellar Formulations of a PEGylated Photosensitizing Phthalocyanine by NMR and Optical Techniques
por: Gergely, Lea P., et al.
Publicado: (2023)

Observations upon Intra-Cranial Tuberculosis in Childhood
por: Branson, William P. S.
Publicado: (1907)

Conformational adaptation of UNCG loops upon crowding
por: Meyer, Mélanie, et al.
Publicado: (2019)

Crossing the rice-wheat border: Not all intra-cultural adaptation is equal
por: English, Alexander S., et al.
Publicado: (2020)

Plant-RNA in Extracellular Vesicles: The Secret of Cross-Kingdom Communication
por: Urzì, Ornella, et al.
Publicado: (2022)

NMR Reveals the Conformational Changes of Cytochrome C upon Interaction with Cardiolipin
por: Zhan, Jianhua, et al.
Publicado: (2021)

Galectins in Intra- and Extracellular Vesicles
por: Bänfer, Sebastian, et al.
Publicado: (2020)

(1)H NMR Spectroscopy Profiling of Metabolic Reprogramming of Chinese Hamster Ovary Cells upon a Temperature Shift during Culture
por: Wagstaff, Jane L., et al.
Publicado: (2013)

ADAPT-NMR Enhancer: complete package for reduced dimensionality in protein NMR spectroscopy
por: Lee, Woonghee, et al.
Publicado: (2013)

Metabolic effects of influenza virus infection in cultured animal cells: Intra- and extracellular metabolite profiling
por: Ritter, Joachim B, et al.
Publicado: (2010)

Iatrogenic Seeding of Tumor Cells in Thigh Soft Tissue Upon Surgical Removal of Intracranial Meningioma
por: Maddah, Ghodratollah, et al.
Publicado: (2016)

Real‐Time NMR Spectroscopy for Studying Metabolism
por: Alshamleh, Islam, et al.
Publicado: (2019)

Robust, Integrated Computational Control of NMR Experiments to Achieve Optimal Assignment by ADAPT-NMR
por: Bahrami, Arash, et al.
Publicado: (2012)

Emerging Insights on the Biological Impact of Extracellular Vesicle-Associated ncRNAs in Multiple Myeloma
por: Raimondo, Stefania, et al.
Publicado: (2020)

Changes in the components of extracellular matrix and in growth properties of cultured aortic smooth muscle cells upon ascorbate feeding
Publicado: (1982)

The challenges of keeping psychiatric patients safe in rehabilitation centers during coronavirus outbreak
por: Maddah, Seyedmahrokh A., et al.
Publicado: (2020)

Three‐dimensional reconstruction of interstitial extracellular vesicles in human liver as determined by electron tomography
por: Olofsson Bagge, Roger, et al.
Publicado: (2023)

L11 domain rearrangement upon binding to RNA and thiostrepton studied by NMR spectroscopy
por: Jonker, Hendrik R. A., et al.
Publicado: (2007)

BcL-xL Conformational Changes upon Fragment Binding Revealed by NMR
por: Aguirre, Clémentine, et al.
Publicado: (2013)

Real-time monitoring of extracellular ATP in bacterial cultures using thermostable luciferase
por: Ihssen, Julian, et al.
Publicado: (2021)

Technical Challenges of Real-Time Adaptive MR-Guided Radiotherapy
por: Thorwarth, Daniela, et al.
Publicado: (2021)

The Effect of Menstrual Disorders upon the Vascularity and Nutrition of the Intra-Ocular Structures
por: Vance, Reuben A.
Publicado: (1872)

Effect of Menstrual Disorders upon the Vascularity and Nutrition of the Intra-ocular Structures
por: Vance, Reuben A.
Publicado: (1872)

Practical Applications of NMR to Solve Real-World Problems
por: Brinson, Robert G.
Publicado: (2021)

A Real-Life Reproducibility Assessment for NMR Metabolomics †
por: Stavarache, Cristina, et al.
Publicado: (2022)

Intra and Extracellular Journey of the Phytohormone Salicylic Acid
por: Maruri-López, Israel, et al.
Publicado: (2019)

Cannot write session to /tmp/vufind_sessions/sess_tbben9506mnoc30t5tvosm0mt7