Ligation-assisted homologous recombination enables precise genome editing by deploying both MMEJ and HDR

CRISPR/Cas12a is a single effector nuclease that, like CRISPR/Cas9, has been harnessed for genome editing based on its ability to generate targeted DNA double strand breaks (DSBs). Unlike the blunt-ended DSB generated by Cas9, Cas12a generates sticky-ended DSB that could potentially aid precise geno...

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Detalles Bibliográficos
Autores principales: Zhao, Zhihan, Shang, Peng, Sage, Fanny, Geijsen, Niels
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2022
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9226534/
https://www.ncbi.nlm.nih.gov/pubmed/35212386
http://dx.doi.org/10.1093/nar/gkac118

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9226534/
https://www.ncbi.nlm.nih.gov/pubmed/35212386
http://dx.doi.org/10.1093/nar/gkac118

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