CRISPR/Cas13 effectors have differing extents of off-target effects that limit their utility in eukaryotic cells

CRISPR/Cas13 effectors have garnered increasing attention as easily customizable tools for detecting and depleting RNAs of interest. Near perfect complementarity between a target RNA and the Cas13-associated guide RNA is required for activation of Cas13 ribonuclease activity. Nonetheless, the specif...

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Detalles Bibliográficos
Autores principales: Ai, Yuxi, Liang, Dongming, Wilusz, Jeremy E
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2022
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9226543/
https://www.ncbi.nlm.nih.gov/pubmed/35244715
http://dx.doi.org/10.1093/nar/gkac159

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9226543/
https://www.ncbi.nlm.nih.gov/pubmed/35244715
http://dx.doi.org/10.1093/nar/gkac159

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