Green Biosynthesis of Selenium Nanoparticles Using Orange Peel Waste: Characterization, Antibacterial and Antibiofilm Activities against Multidrug-Resistant Bacteria

There is an increase of pathogenic multidrug-resistant bacteria globally due to the misuse of antibiotics. Recently, more scientists used metal nanoparticles to counteract antibacterial resistance. In this study, orange peel waste (OPW) was used for selenium nanoparticles’ (Se-NPs) biosynthesis thro...

Descripción completa

Detalles Bibliográficos
Autores principales: Salem, Salem S., Badawy, Mona Shaban E. M., Al-Askar, Abdulaziz A., Arishi, Amr Abker, Elkady, Fathy M., Hashem, Amr H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9227136/
https://www.ncbi.nlm.nih.gov/pubmed/35743924
http://dx.doi.org/10.3390/life12060893
_version_ 1784734089133162496
author Salem, Salem S.
Badawy, Mona Shaban E. M.
Al-Askar, Abdulaziz A.
Arishi, Amr Abker
Elkady, Fathy M.
Hashem, Amr H.
author_facet Salem, Salem S.
Badawy, Mona Shaban E. M.
Al-Askar, Abdulaziz A.
Arishi, Amr Abker
Elkady, Fathy M.
Hashem, Amr H.
author_sort Salem, Salem S.
collection PubMed
description There is an increase of pathogenic multidrug-resistant bacteria globally due to the misuse of antibiotics. Recently, more scientists used metal nanoparticles to counteract antibacterial resistance. In this study, orange peel waste (OPW) was used for selenium nanoparticles’ (Se-NPs) biosynthesis through the green and ecofriendly method, and their applications as antibacterial and antibiofilm agents. Green biosynthesized Se-NPs were characterized using FTIR, XRD, SEM, EDAX, and TEM. Characterization results revealed that biosynthesized Se-NPs were highly crystalline, spherical, and polydisperse, and had sizes in the range of 16–95 nm. The biosynthesized Se-NPs were evaluated as antibacterial and antibiofilm activities against multidrug-resistant bacteria. Results illustrated that Se-NPs exhibited potential antibacterial activity against Gram-positive bacteria (S. aureus ATCC 29213 and biofilm-producing clinical isolates of S. aureus) and Gram-negative bacteria (Pseudomonas aeruginosa PAO1, MDR, biofilm, and quorum-sensing and producing clinical isolates of MDR P. aeruginosa, MDR E. coli, and K. pneumonia). Moreover, results illustrated that S. aureus ATCC 29213 was the most sensitive bacteria to Se-NPs at 1000 µg/mL, where the inhibition zone was 35 mm and MIC was 25 µg/mL. Furthermore, Se-NPs at 0.25 and 0.5 MIC decreased the biofilm significantly. The largest inhibition of biofilm was noticed in MDR K. pneumonia, which was 62% and 92% at 0.25 and 0.5 MIC, respectively. In conclusion, Se-NPs were successfully biosynthesized using OPW through the green method and had promising antibacterial and antibiofilm activity against multidrug-resistant bacteria, which can be used later in fighting resistant bacteria.
format Online
Article
Text
id pubmed-9227136
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-92271362022-06-25 Green Biosynthesis of Selenium Nanoparticles Using Orange Peel Waste: Characterization, Antibacterial and Antibiofilm Activities against Multidrug-Resistant Bacteria Salem, Salem S. Badawy, Mona Shaban E. M. Al-Askar, Abdulaziz A. Arishi, Amr Abker Elkady, Fathy M. Hashem, Amr H. Life (Basel) Article There is an increase of pathogenic multidrug-resistant bacteria globally due to the misuse of antibiotics. Recently, more scientists used metal nanoparticles to counteract antibacterial resistance. In this study, orange peel waste (OPW) was used for selenium nanoparticles’ (Se-NPs) biosynthesis through the green and ecofriendly method, and their applications as antibacterial and antibiofilm agents. Green biosynthesized Se-NPs were characterized using FTIR, XRD, SEM, EDAX, and TEM. Characterization results revealed that biosynthesized Se-NPs were highly crystalline, spherical, and polydisperse, and had sizes in the range of 16–95 nm. The biosynthesized Se-NPs were evaluated as antibacterial and antibiofilm activities against multidrug-resistant bacteria. Results illustrated that Se-NPs exhibited potential antibacterial activity against Gram-positive bacteria (S. aureus ATCC 29213 and biofilm-producing clinical isolates of S. aureus) and Gram-negative bacteria (Pseudomonas aeruginosa PAO1, MDR, biofilm, and quorum-sensing and producing clinical isolates of MDR P. aeruginosa, MDR E. coli, and K. pneumonia). Moreover, results illustrated that S. aureus ATCC 29213 was the most sensitive bacteria to Se-NPs at 1000 µg/mL, where the inhibition zone was 35 mm and MIC was 25 µg/mL. Furthermore, Se-NPs at 0.25 and 0.5 MIC decreased the biofilm significantly. The largest inhibition of biofilm was noticed in MDR K. pneumonia, which was 62% and 92% at 0.25 and 0.5 MIC, respectively. In conclusion, Se-NPs were successfully biosynthesized using OPW through the green method and had promising antibacterial and antibiofilm activity against multidrug-resistant bacteria, which can be used later in fighting resistant bacteria. MDPI 2022-06-15 /pmc/articles/PMC9227136/ /pubmed/35743924 http://dx.doi.org/10.3390/life12060893 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Salem, Salem S.
Badawy, Mona Shaban E. M.
Al-Askar, Abdulaziz A.
Arishi, Amr Abker
Elkady, Fathy M.
Hashem, Amr H.
Green Biosynthesis of Selenium Nanoparticles Using Orange Peel Waste: Characterization, Antibacterial and Antibiofilm Activities against Multidrug-Resistant Bacteria
title Green Biosynthesis of Selenium Nanoparticles Using Orange Peel Waste: Characterization, Antibacterial and Antibiofilm Activities against Multidrug-Resistant Bacteria
title_full Green Biosynthesis of Selenium Nanoparticles Using Orange Peel Waste: Characterization, Antibacterial and Antibiofilm Activities against Multidrug-Resistant Bacteria
title_fullStr Green Biosynthesis of Selenium Nanoparticles Using Orange Peel Waste: Characterization, Antibacterial and Antibiofilm Activities against Multidrug-Resistant Bacteria
title_full_unstemmed Green Biosynthesis of Selenium Nanoparticles Using Orange Peel Waste: Characterization, Antibacterial and Antibiofilm Activities against Multidrug-Resistant Bacteria
title_short Green Biosynthesis of Selenium Nanoparticles Using Orange Peel Waste: Characterization, Antibacterial and Antibiofilm Activities against Multidrug-Resistant Bacteria
title_sort green biosynthesis of selenium nanoparticles using orange peel waste: characterization, antibacterial and antibiofilm activities against multidrug-resistant bacteria
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9227136/
https://www.ncbi.nlm.nih.gov/pubmed/35743924
http://dx.doi.org/10.3390/life12060893
work_keys_str_mv AT salemsalems greenbiosynthesisofseleniumnanoparticlesusingorangepeelwastecharacterizationantibacterialandantibiofilmactivitiesagainstmultidrugresistantbacteria
AT badawymonashabanem greenbiosynthesisofseleniumnanoparticlesusingorangepeelwastecharacterizationantibacterialandantibiofilmactivitiesagainstmultidrugresistantbacteria
AT alaskarabdulaziza greenbiosynthesisofseleniumnanoparticlesusingorangepeelwastecharacterizationantibacterialandantibiofilmactivitiesagainstmultidrugresistantbacteria
AT arishiamrabker greenbiosynthesisofseleniumnanoparticlesusingorangepeelwastecharacterizationantibacterialandantibiofilmactivitiesagainstmultidrugresistantbacteria
AT elkadyfathym greenbiosynthesisofseleniumnanoparticlesusingorangepeelwastecharacterizationantibacterialandantibiofilmactivitiesagainstmultidrugresistantbacteria
AT hashemamrh greenbiosynthesisofseleniumnanoparticlesusingorangepeelwastecharacterizationantibacterialandantibiofilmactivitiesagainstmultidrugresistantbacteria