Measurement of Tetrahydrobiopterin in Animal Tissue Samples by HPLC with Electrochemical Detection—Protocol Optimization and Pitfalls

Tetrahydrobiopterin (BH4) is an essential cofactor of all nitric oxide synthase isoforms, thus determination of BH4 levels can provide important mechanistic insight into diseases. We established a protocol for high-performance liquid chromatography/electrochemical detection (HPLC/ECD)-based determin...

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Detalles Bibliográficos
Autores principales: Vujacic-Mirski, Ksenija, Oelze, Matthias, Kuntic, Ivana, Kuntic, Marin, Kalinovic, Sanela, Li, Huige, Zielonka, Jacek, Münzel, Thomas, Daiber, Andreas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9228106/
https://www.ncbi.nlm.nih.gov/pubmed/35740082
http://dx.doi.org/10.3390/antiox11061182

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9228106/
https://www.ncbi.nlm.nih.gov/pubmed/35740082
http://dx.doi.org/10.3390/antiox11061182

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