Validation of a Novel Double Control Quantitative Copy Number PCR Method to Quantify Off-Target Transgene Integration after CRISPR-Induced DNA Modification

In order to improve a recently established cell-based assay to assess the potency of botulinum neurotoxin, neuroblastoma-derived SiMa cells and induced pluripotent stem-cells (iPSC) were modified to incorporate the coding sequence of a reporter luciferase into a genetic safe harbor utilizing CRISPR/...

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Detalles Bibliográficos
Autores principales: Schjeide, Brit-Maren Michaud, Schenke, Maren, Seeger, Bettina, Püschel, Gerhard Paul
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9229199/
https://www.ncbi.nlm.nih.gov/pubmed/35736544
http://dx.doi.org/10.3390/mps5030043

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9229199/
https://www.ncbi.nlm.nih.gov/pubmed/35736544
http://dx.doi.org/10.3390/mps5030043

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