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Analysis of commonly expressed genes between first trimester fetal heart and placenta cell types in the context of congenital heart disease

Congenital heart disease (CHD) is often associated with fetal growth abnormalities. During the first trimester of pregnancy, the heart and placenta develop concurrently, and share key developmental pathways. It is hypothesized that defective morphogenesis of either organ is synergistically linked. H...

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Autores principales: Wilson, Rebecca L., Yuan, Victor, Courtney, Jennifer A., Tipler, Alyssa, Cnota, James F., Jones, Helen N.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9232495/
https://www.ncbi.nlm.nih.gov/pubmed/35750800
http://dx.doi.org/10.1038/s41598-022-14955-8
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author Wilson, Rebecca L.
Yuan, Victor
Courtney, Jennifer A.
Tipler, Alyssa
Cnota, James F.
Jones, Helen N.
author_facet Wilson, Rebecca L.
Yuan, Victor
Courtney, Jennifer A.
Tipler, Alyssa
Cnota, James F.
Jones, Helen N.
author_sort Wilson, Rebecca L.
collection PubMed
description Congenital heart disease (CHD) is often associated with fetal growth abnormalities. During the first trimester of pregnancy, the heart and placenta develop concurrently, and share key developmental pathways. It is hypothesized that defective morphogenesis of either organ is synergistically linked. However, many studies determined to understand the mechanisms behind CHD overlook the contribution of the placenta. In this study, we aimed to identify commonly expressed genes between first trimester heart and placenta cells using two publicly available single cell sequencing databases. Using a systematic computational approach, we identified 328 commonly expressed genes between heart and placenta endothelial cells and enrichment in pathways including Vasculature Development (GO:0001944, FDR 2.90E−30), and Angiogenesis (GO:0001525, FDR 1.18E−27). We also found, in comparison with fetal heart endothelial cells, 197 commonly expressed genes with placenta extravillous trophoblasts, 128 with cytotrophoblasts and 80 with syncytiotrophoblasts, and included genes such as FLT1, GATA2, ENG and CDH5. Finally, comparison of first trimester cardiomyocytes and placenta cytotrophoblasts revealed 53 commonly expressed genes and enrichment in biological processes integral to cellular function including Cellular Respiration (GO:0045333; FDR 5.05E−08), Ion Transport (GO:0006811; FDR 2.08E−02), and Oxidation–Reduction Process (GO:0055114; FDR 1.58E−07). Overall, our results identify specific genes and cellular pathways common between first trimester fetal heart and placenta cells which if disrupted may concurrently contribute to the developmental perturbations resulting in CHD.
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spelling pubmed-92324952022-06-26 Analysis of commonly expressed genes between first trimester fetal heart and placenta cell types in the context of congenital heart disease Wilson, Rebecca L. Yuan, Victor Courtney, Jennifer A. Tipler, Alyssa Cnota, James F. Jones, Helen N. Sci Rep Article Congenital heart disease (CHD) is often associated with fetal growth abnormalities. During the first trimester of pregnancy, the heart and placenta develop concurrently, and share key developmental pathways. It is hypothesized that defective morphogenesis of either organ is synergistically linked. However, many studies determined to understand the mechanisms behind CHD overlook the contribution of the placenta. In this study, we aimed to identify commonly expressed genes between first trimester heart and placenta cells using two publicly available single cell sequencing databases. Using a systematic computational approach, we identified 328 commonly expressed genes between heart and placenta endothelial cells and enrichment in pathways including Vasculature Development (GO:0001944, FDR 2.90E−30), and Angiogenesis (GO:0001525, FDR 1.18E−27). We also found, in comparison with fetal heart endothelial cells, 197 commonly expressed genes with placenta extravillous trophoblasts, 128 with cytotrophoblasts and 80 with syncytiotrophoblasts, and included genes such as FLT1, GATA2, ENG and CDH5. Finally, comparison of first trimester cardiomyocytes and placenta cytotrophoblasts revealed 53 commonly expressed genes and enrichment in biological processes integral to cellular function including Cellular Respiration (GO:0045333; FDR 5.05E−08), Ion Transport (GO:0006811; FDR 2.08E−02), and Oxidation–Reduction Process (GO:0055114; FDR 1.58E−07). Overall, our results identify specific genes and cellular pathways common between first trimester fetal heart and placenta cells which if disrupted may concurrently contribute to the developmental perturbations resulting in CHD. Nature Publishing Group UK 2022-06-24 /pmc/articles/PMC9232495/ /pubmed/35750800 http://dx.doi.org/10.1038/s41598-022-14955-8 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Wilson, Rebecca L.
Yuan, Victor
Courtney, Jennifer A.
Tipler, Alyssa
Cnota, James F.
Jones, Helen N.
Analysis of commonly expressed genes between first trimester fetal heart and placenta cell types in the context of congenital heart disease
title Analysis of commonly expressed genes between first trimester fetal heart and placenta cell types in the context of congenital heart disease
title_full Analysis of commonly expressed genes between first trimester fetal heart and placenta cell types in the context of congenital heart disease
title_fullStr Analysis of commonly expressed genes between first trimester fetal heart and placenta cell types in the context of congenital heart disease
title_full_unstemmed Analysis of commonly expressed genes between first trimester fetal heart and placenta cell types in the context of congenital heart disease
title_short Analysis of commonly expressed genes between first trimester fetal heart and placenta cell types in the context of congenital heart disease
title_sort analysis of commonly expressed genes between first trimester fetal heart and placenta cell types in the context of congenital heart disease
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9232495/
https://www.ncbi.nlm.nih.gov/pubmed/35750800
http://dx.doi.org/10.1038/s41598-022-14955-8
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