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The STING1-MYD88 complex drives ACOD1/IRG1 expression and function in lethal innate immunity
ACOD1 (also known as IRG1) has emerged as a regulator of immunometabolism that operates by producing metabolite itaconate. Here, we report a key role of STING1 (also known as STING and TMEM173) in mediating ACOD1 expression in myeloid cells in response to toll-like receptor (TLR) signaling. The acti...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9234224/ https://www.ncbi.nlm.nih.gov/pubmed/35769880 http://dx.doi.org/10.1016/j.isci.2022.104561 |
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author | Chen, Feng Wu, Runliu Liu, Jiao Kang, Rui Li, Jinbao Tang, Daolin |
author_facet | Chen, Feng Wu, Runliu Liu, Jiao Kang, Rui Li, Jinbao Tang, Daolin |
author_sort | Chen, Feng |
collection | PubMed |
description | ACOD1 (also known as IRG1) has emerged as a regulator of immunometabolism that operates by producing metabolite itaconate. Here, we report a key role of STING1 (also known as STING and TMEM173) in mediating ACOD1 expression in myeloid cells in response to toll-like receptor (TLR) signaling. The activation of STING1 through exogenous cyclic dinucleotides (e.g., 3′3′-cGAMP) or endogenous gain-of-function mutation (e.g., V155M) enhances lipopolysaccharide-induced ACOD1 expression and itaconate production in macrophages and monocytes, whereas the deletion of STING1 blocks this process. The adaptor protein MYD88, instead of DNA sensor cyclic GMP-AMP synthase (CGAS), favors STING1-dependent ACOD1 expression. Mechanistically, MYD88 directly blocks autophagic degradation of STING1 and causes subsequent IRF3/JUN-mediated ACOD1 gene transcription. Consequently, the conditional deletion of STING1 in myeloid cells fails to produce ACOD1 and itaconate, thereby protecting mice against endotoxemia and polymicrobial sepsis. Our results, therefore, establish a direct link between TLR4 signaling and ACOD1 expression through the STING1-MYD88 complex during septic shock. |
format | Online Article Text |
id | pubmed-9234224 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-92342242022-06-28 The STING1-MYD88 complex drives ACOD1/IRG1 expression and function in lethal innate immunity Chen, Feng Wu, Runliu Liu, Jiao Kang, Rui Li, Jinbao Tang, Daolin iScience Article ACOD1 (also known as IRG1) has emerged as a regulator of immunometabolism that operates by producing metabolite itaconate. Here, we report a key role of STING1 (also known as STING and TMEM173) in mediating ACOD1 expression in myeloid cells in response to toll-like receptor (TLR) signaling. The activation of STING1 through exogenous cyclic dinucleotides (e.g., 3′3′-cGAMP) or endogenous gain-of-function mutation (e.g., V155M) enhances lipopolysaccharide-induced ACOD1 expression and itaconate production in macrophages and monocytes, whereas the deletion of STING1 blocks this process. The adaptor protein MYD88, instead of DNA sensor cyclic GMP-AMP synthase (CGAS), favors STING1-dependent ACOD1 expression. Mechanistically, MYD88 directly blocks autophagic degradation of STING1 and causes subsequent IRF3/JUN-mediated ACOD1 gene transcription. Consequently, the conditional deletion of STING1 in myeloid cells fails to produce ACOD1 and itaconate, thereby protecting mice against endotoxemia and polymicrobial sepsis. Our results, therefore, establish a direct link between TLR4 signaling and ACOD1 expression through the STING1-MYD88 complex during septic shock. Elsevier 2022-06-08 /pmc/articles/PMC9234224/ /pubmed/35769880 http://dx.doi.org/10.1016/j.isci.2022.104561 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Chen, Feng Wu, Runliu Liu, Jiao Kang, Rui Li, Jinbao Tang, Daolin The STING1-MYD88 complex drives ACOD1/IRG1 expression and function in lethal innate immunity |
title | The STING1-MYD88 complex drives ACOD1/IRG1 expression and function in lethal innate immunity |
title_full | The STING1-MYD88 complex drives ACOD1/IRG1 expression and function in lethal innate immunity |
title_fullStr | The STING1-MYD88 complex drives ACOD1/IRG1 expression and function in lethal innate immunity |
title_full_unstemmed | The STING1-MYD88 complex drives ACOD1/IRG1 expression and function in lethal innate immunity |
title_short | The STING1-MYD88 complex drives ACOD1/IRG1 expression and function in lethal innate immunity |
title_sort | sting1-myd88 complex drives acod1/irg1 expression and function in lethal innate immunity |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9234224/ https://www.ncbi.nlm.nih.gov/pubmed/35769880 http://dx.doi.org/10.1016/j.isci.2022.104561 |
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