Protocol to study sufficiency of cis-regulatory elements in mouse embryonic stem cells using a CRISPR-mediated knockin approach

cis-regulatory elements (CREs) orchestrate the spatiotemporal control of gene expression. The regulatory activity of CREs is typically assessed by reporter assays, in which CREs are studied outside their endogenous context. To circumvent this problem, we developed a CRISPR-Cas9 knockin approach to s...

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Detalles Bibliográficos
Autores principales: Pachano, Tomas, Rada-Iglesias, Alvaro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9234469/
https://www.ncbi.nlm.nih.gov/pubmed/35769926
http://dx.doi.org/10.1016/j.xpro.2022.101492

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9234469/
https://www.ncbi.nlm.nih.gov/pubmed/35769926
http://dx.doi.org/10.1016/j.xpro.2022.101492

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