Lipofection of Non-integrative CRISPR/Cas9 Ribonucleoproteins in Male Germline Stem Cells: A Simple and Effective Knockout Tool for Germline Genome Engineering

Gene editing in male germline stem (GS) cells is a potent tool to study spermatogenesis and to create transgenic mice. Various engineered nucleases already demonstrated the ability to modify the genome of GS cells. However, current systems are limited by technical complexity diminishing application...

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Detalles Bibliográficos
Autores principales: Obermeier, Mariella, Vadolas, Jim, Verhulst, Stefaan, Goossens, Ellen, Baert, Yoni
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Cell and Developmental Biology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9237505/
https://www.ncbi.nlm.nih.gov/pubmed/35774227
http://dx.doi.org/10.3389/fcell.2022.891173

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9237505/
https://www.ncbi.nlm.nih.gov/pubmed/35774227
http://dx.doi.org/10.3389/fcell.2022.891173

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