Traditional protocols and optimization methods lead to absent expression in a mycoplasma cell-free gene expression platform

Cell-free expression (CFE) systems are one of the main platforms for building synthetic cells. A major drawback is the orthogonality of cell-free systems across species. To generate a CFE system compatible with recently established minimal cell constructs, we attempted to optimize a Mycoplasma bacte...

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Detalles Bibliográficos
Autores principales: Sakai, Andrei, Deich, Christopher R, Nelissen, Frank H T, Jonker, Aafke J, Bittencourt, Daniela M de C, Kempes, Christopher P, Wise, Kim S, Heus, Hans A, Huck, Wilhelm T S, Adamala, Katarzyna P, Glass, John I
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2022
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9239315/
https://www.ncbi.nlm.nih.gov/pubmed/35774105
http://dx.doi.org/10.1093/synbio/ysac008

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9239315/
https://www.ncbi.nlm.nih.gov/pubmed/35774105
http://dx.doi.org/10.1093/synbio/ysac008

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