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The Bridging Effect of Controlled-Release Glial Cell-Derived Neurotrophic Factor Microcapsules within Nerve Conduits on Rat Facial Nerve Regeneration

OBJECTIVES: The study is aimed at exploring the effect of the controlled release of the glial-derived neurotrophic factor (GDNF) on nerve regeneration. METHODS: The PLGA/chitosan composite nerve conduit was used to bridge the dissected trunk of the rat facial nerve. GDNF microcapsules were loaded in...

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Autores principales: Xia, Siwen, Zhang, Mingxing, Li, Meng, Song, Xianmin, Chen, Donghui, Zhu, Minhui, Zheng, Hongliang, Chen, Shicai
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9239766/
https://www.ncbi.nlm.nih.gov/pubmed/35774850
http://dx.doi.org/10.1155/2022/8942985
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author Xia, Siwen
Zhang, Mingxing
Li, Meng
Song, Xianmin
Chen, Donghui
Zhu, Minhui
Zheng, Hongliang
Chen, Shicai
author_facet Xia, Siwen
Zhang, Mingxing
Li, Meng
Song, Xianmin
Chen, Donghui
Zhu, Minhui
Zheng, Hongliang
Chen, Shicai
author_sort Xia, Siwen
collection PubMed
description OBJECTIVES: The study is aimed at exploring the effect of the controlled release of the glial-derived neurotrophic factor (GDNF) on nerve regeneration. METHODS: The PLGA/chitosan composite nerve conduit was used to bridge the dissected trunk of the rat facial nerve. GDNF microcapsules were loaded into the nerve conduit. Nine weeks after surgery, the facial nerve zygomatic and buccal branches were labeled with fluorescent indicators. The incorrectly grown facial neurons were reversed and counted. The facial nerve functional recovery was assessed by measuring the maximum evoked potential. RESULTS: The nerve conduit was filled with different regenerating factors, such as the GDNF, GDNF microcapsules, or saline (control). The number of incorrectly regenerated neurons was lower with the nerve conduits filled with GDNF microcapsules than with those supplied with just the GDNF. However, neither the GDNF nor GDNF microcapsules affected the number of regenerated neurons. The functional recovery of the facial nerve was the best, with the nerve conduit filled with GDNF microcapsules closest to the healthy uncut facial nerve. CONCLUSION: The stable slow-release GNDF microcapsule inside the biodegradable nerve conduit can reduce the extent of incorrect growth of the facial nerve neuron when bridging the dissected rat facial nerve trunk. The technique has a good effect on functional nerve recovery.
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spelling pubmed-92397662022-06-29 The Bridging Effect of Controlled-Release Glial Cell-Derived Neurotrophic Factor Microcapsules within Nerve Conduits on Rat Facial Nerve Regeneration Xia, Siwen Zhang, Mingxing Li, Meng Song, Xianmin Chen, Donghui Zhu, Minhui Zheng, Hongliang Chen, Shicai Dis Markers Research Article OBJECTIVES: The study is aimed at exploring the effect of the controlled release of the glial-derived neurotrophic factor (GDNF) on nerve regeneration. METHODS: The PLGA/chitosan composite nerve conduit was used to bridge the dissected trunk of the rat facial nerve. GDNF microcapsules were loaded into the nerve conduit. Nine weeks after surgery, the facial nerve zygomatic and buccal branches were labeled with fluorescent indicators. The incorrectly grown facial neurons were reversed and counted. The facial nerve functional recovery was assessed by measuring the maximum evoked potential. RESULTS: The nerve conduit was filled with different regenerating factors, such as the GDNF, GDNF microcapsules, or saline (control). The number of incorrectly regenerated neurons was lower with the nerve conduits filled with GDNF microcapsules than with those supplied with just the GDNF. However, neither the GDNF nor GDNF microcapsules affected the number of regenerated neurons. The functional recovery of the facial nerve was the best, with the nerve conduit filled with GDNF microcapsules closest to the healthy uncut facial nerve. CONCLUSION: The stable slow-release GNDF microcapsule inside the biodegradable nerve conduit can reduce the extent of incorrect growth of the facial nerve neuron when bridging the dissected rat facial nerve trunk. The technique has a good effect on functional nerve recovery. Hindawi 2022-06-21 /pmc/articles/PMC9239766/ /pubmed/35774850 http://dx.doi.org/10.1155/2022/8942985 Text en Copyright © 2022 Siwen Xia et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Xia, Siwen
Zhang, Mingxing
Li, Meng
Song, Xianmin
Chen, Donghui
Zhu, Minhui
Zheng, Hongliang
Chen, Shicai
The Bridging Effect of Controlled-Release Glial Cell-Derived Neurotrophic Factor Microcapsules within Nerve Conduits on Rat Facial Nerve Regeneration
title The Bridging Effect of Controlled-Release Glial Cell-Derived Neurotrophic Factor Microcapsules within Nerve Conduits on Rat Facial Nerve Regeneration
title_full The Bridging Effect of Controlled-Release Glial Cell-Derived Neurotrophic Factor Microcapsules within Nerve Conduits on Rat Facial Nerve Regeneration
title_fullStr The Bridging Effect of Controlled-Release Glial Cell-Derived Neurotrophic Factor Microcapsules within Nerve Conduits on Rat Facial Nerve Regeneration
title_full_unstemmed The Bridging Effect of Controlled-Release Glial Cell-Derived Neurotrophic Factor Microcapsules within Nerve Conduits on Rat Facial Nerve Regeneration
title_short The Bridging Effect of Controlled-Release Glial Cell-Derived Neurotrophic Factor Microcapsules within Nerve Conduits on Rat Facial Nerve Regeneration
title_sort bridging effect of controlled-release glial cell-derived neurotrophic factor microcapsules within nerve conduits on rat facial nerve regeneration
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9239766/
https://www.ncbi.nlm.nih.gov/pubmed/35774850
http://dx.doi.org/10.1155/2022/8942985
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