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A model-specific simplification of the Mouse Grimace Scale based on the pain response of intraperitoneal CCl(4) injections

Despite its long establishment and applicability in mice pain detection, the Mouse Grimace Scale still seems to be underused in acute pain detection during chronic experiments. However, broadening its applicability can identify possible refinement approaches such as cumulative severity and habituati...

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Autores principales: Ernst, Lisa, Bruch, Stefan, Kopaczka, Marcin, Merhof, Dorit, Bleich, André, Tolba, René H., Talbot, Steven R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9240072/
https://www.ncbi.nlm.nih.gov/pubmed/35764784
http://dx.doi.org/10.1038/s41598-022-14852-0
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author Ernst, Lisa
Bruch, Stefan
Kopaczka, Marcin
Merhof, Dorit
Bleich, André
Tolba, René H.
Talbot, Steven R.
author_facet Ernst, Lisa
Bruch, Stefan
Kopaczka, Marcin
Merhof, Dorit
Bleich, André
Tolba, René H.
Talbot, Steven R.
author_sort Ernst, Lisa
collection PubMed
description Despite its long establishment and applicability in mice pain detection, the Mouse Grimace Scale still seems to be underused in acute pain detection during chronic experiments. However, broadening its applicability can identify possible refinement approaches such as cumulative severity and habituation to painful stimuli. Therefore, this study focuses on two main aspects: First, five composite MGS criteria were evaluated with two independent methods (the MoBPs algorithm and a penalized least squares regression) and ranked for their relative importance. The most important variable was used in a second analysis to specifically evaluate the context of pain after an i.p. injection (intervention) in two treatment groups (CCl(4) and oil (control)) at fixed times throughout four weeks in 24 male C57BL/6 N mice. One hour before and after each intervention, video recordings were taken, and the MGS assessment was performed. In this study, the results indicate orbital tightening as the most important criterion. In this experimental setup, a highly significant difference after treatment between week 0 and 1 was found in the CCl(4) group, resulting in a medium-sized effect (W = 62.5, p value < 0.0001, r(CCl4) = 0.64). The oil group showed no significant difference (week 0 vs 1, W = 291.5, p value = 0.7875, r(control) = 0.04). Therefore, the study showed that the pain caused by i.p. injections was only dependent on the applied substance, and no significant cumulation or habituation occurred due to the intervention. Further, the results indicated that the MGS system can be simplified.
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spelling pubmed-92400722022-06-30 A model-specific simplification of the Mouse Grimace Scale based on the pain response of intraperitoneal CCl(4) injections Ernst, Lisa Bruch, Stefan Kopaczka, Marcin Merhof, Dorit Bleich, André Tolba, René H. Talbot, Steven R. Sci Rep Article Despite its long establishment and applicability in mice pain detection, the Mouse Grimace Scale still seems to be underused in acute pain detection during chronic experiments. However, broadening its applicability can identify possible refinement approaches such as cumulative severity and habituation to painful stimuli. Therefore, this study focuses on two main aspects: First, five composite MGS criteria were evaluated with two independent methods (the MoBPs algorithm and a penalized least squares regression) and ranked for their relative importance. The most important variable was used in a second analysis to specifically evaluate the context of pain after an i.p. injection (intervention) in two treatment groups (CCl(4) and oil (control)) at fixed times throughout four weeks in 24 male C57BL/6 N mice. One hour before and after each intervention, video recordings were taken, and the MGS assessment was performed. In this study, the results indicate orbital tightening as the most important criterion. In this experimental setup, a highly significant difference after treatment between week 0 and 1 was found in the CCl(4) group, resulting in a medium-sized effect (W = 62.5, p value < 0.0001, r(CCl4) = 0.64). The oil group showed no significant difference (week 0 vs 1, W = 291.5, p value = 0.7875, r(control) = 0.04). Therefore, the study showed that the pain caused by i.p. injections was only dependent on the applied substance, and no significant cumulation or habituation occurred due to the intervention. Further, the results indicated that the MGS system can be simplified. Nature Publishing Group UK 2022-06-28 /pmc/articles/PMC9240072/ /pubmed/35764784 http://dx.doi.org/10.1038/s41598-022-14852-0 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Ernst, Lisa
Bruch, Stefan
Kopaczka, Marcin
Merhof, Dorit
Bleich, André
Tolba, René H.
Talbot, Steven R.
A model-specific simplification of the Mouse Grimace Scale based on the pain response of intraperitoneal CCl(4) injections
title A model-specific simplification of the Mouse Grimace Scale based on the pain response of intraperitoneal CCl(4) injections
title_full A model-specific simplification of the Mouse Grimace Scale based on the pain response of intraperitoneal CCl(4) injections
title_fullStr A model-specific simplification of the Mouse Grimace Scale based on the pain response of intraperitoneal CCl(4) injections
title_full_unstemmed A model-specific simplification of the Mouse Grimace Scale based on the pain response of intraperitoneal CCl(4) injections
title_short A model-specific simplification of the Mouse Grimace Scale based on the pain response of intraperitoneal CCl(4) injections
title_sort model-specific simplification of the mouse grimace scale based on the pain response of intraperitoneal ccl(4) injections
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9240072/
https://www.ncbi.nlm.nih.gov/pubmed/35764784
http://dx.doi.org/10.1038/s41598-022-14852-0
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