SpySwitch enables pH- or heat-responsive capture and release for plug-and-display nanoassembly

Proteins can be empowered via SpyTag for anchoring and nanoassembly, through covalent bonding to SpyCatcher partners. Here we generate a switchable version of SpyCatcher, allowing gentle purification of SpyTagged proteins. We introduce numerous histidines adjacent to SpyTag’s binding site, giving mo...

Descripción completa

Detalles Bibliográficos
Autores principales: Vester, Susan K., Rahikainen, Rolle, Khairil Anuar, Irsyad N. A., Hills, Rory A., Tan, Tiong Kit, Howarth, Mark
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9240080/
https://www.ncbi.nlm.nih.gov/pubmed/35764623
http://dx.doi.org/10.1038/s41467-022-31193-8

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9240080/
https://www.ncbi.nlm.nih.gov/pubmed/35764623
http://dx.doi.org/10.1038/s41467-022-31193-8

Ejemplares similares