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SpySwitch enables pH- or heat-responsive capture and release for plug-and-display nanoassembly

Proteins can be empowered via SpyTag for anchoring and nanoassembly, through covalent bonding to SpyCatcher partners. Here we generate a switchable version of SpyCatcher, allowing gentle purification of SpyTagged proteins. We introduce numerous histidines adjacent to SpyTag’s binding site, giving mo...

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Detalles Bibliográficos
Autores principales:	Vester, Susan K., Rahikainen, Rolle, Khairil Anuar, Irsyad N. A., Hills, Rory A., Tan, Tiong Kit, Howarth, Mark
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group UK 2022
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9240080/ https://www.ncbi.nlm.nih.gov/pubmed/35764623 http://dx.doi.org/10.1038/s41467-022-31193-8

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