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Effects of Hydroxyurea on Skeletal Muscle Energetics and Function in a Mildly Anemic Mouse Model

Hydroxyurea (HU) is a ribonucleotide reductase inhibitor most commonly used as a therapeutic agent in sickle cell disease (SCD) with the aim of reducing the risk of vaso-occlusion and improving oxygen transport to tissues. Previous studies suggest that HU may be even beneficial in mild anemia. Howev...

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Autores principales: Michel, Constance P., Messonnier, Laurent A., Giannesini, Benoit, Chatel, Benjamin, Vilmen, Christophe, Le Fur, Yann, Bendahan, David
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9240423/
https://www.ncbi.nlm.nih.gov/pubmed/35784862
http://dx.doi.org/10.3389/fphys.2022.915640
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author Michel, Constance P.
Messonnier, Laurent A.
Giannesini, Benoit
Chatel, Benjamin
Vilmen, Christophe
Le Fur, Yann
Bendahan, David
author_facet Michel, Constance P.
Messonnier, Laurent A.
Giannesini, Benoit
Chatel, Benjamin
Vilmen, Christophe
Le Fur, Yann
Bendahan, David
author_sort Michel, Constance P.
collection PubMed
description Hydroxyurea (HU) is a ribonucleotide reductase inhibitor most commonly used as a therapeutic agent in sickle cell disease (SCD) with the aim of reducing the risk of vaso-occlusion and improving oxygen transport to tissues. Previous studies suggest that HU may be even beneficial in mild anemia. However, the corresponding effects on skeletal muscle energetics and function have never been reported in such a mild anemia model. Seventeen mildly anemic HbAA Townes mice were subjected to a standardized rest-stimulation (transcutaneous stimulation)-protocol while muscle energetics using (31)Phosphorus magnetic resonance spectroscopy and muscle force production were assessed and recorded. Eight mice were supplemented with hydroxyurea (HU) for 6 weeks while 9 were not (CON). HU mice displayed a higher specific total force production compared to the CON, with 501.35 ± 54.12 N/mm(3) and 437.43 ± 57.10 N/mm(3) respectively (+14.6%, p < 0.05). Neither the total rate of energy consumption nor the oxidative metabolic rate were significantly different between groups. The present results illustrated a positive effect of a HU chronic supplementation on skeletal muscle function in mice with mild anemia.
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spelling pubmed-92404232022-06-30 Effects of Hydroxyurea on Skeletal Muscle Energetics and Function in a Mildly Anemic Mouse Model Michel, Constance P. Messonnier, Laurent A. Giannesini, Benoit Chatel, Benjamin Vilmen, Christophe Le Fur, Yann Bendahan, David Front Physiol Physiology Hydroxyurea (HU) is a ribonucleotide reductase inhibitor most commonly used as a therapeutic agent in sickle cell disease (SCD) with the aim of reducing the risk of vaso-occlusion and improving oxygen transport to tissues. Previous studies suggest that HU may be even beneficial in mild anemia. However, the corresponding effects on skeletal muscle energetics and function have never been reported in such a mild anemia model. Seventeen mildly anemic HbAA Townes mice were subjected to a standardized rest-stimulation (transcutaneous stimulation)-protocol while muscle energetics using (31)Phosphorus magnetic resonance spectroscopy and muscle force production were assessed and recorded. Eight mice were supplemented with hydroxyurea (HU) for 6 weeks while 9 were not (CON). HU mice displayed a higher specific total force production compared to the CON, with 501.35 ± 54.12 N/mm(3) and 437.43 ± 57.10 N/mm(3) respectively (+14.6%, p < 0.05). Neither the total rate of energy consumption nor the oxidative metabolic rate were significantly different between groups. The present results illustrated a positive effect of a HU chronic supplementation on skeletal muscle function in mice with mild anemia. Frontiers Media S.A. 2022-06-15 /pmc/articles/PMC9240423/ /pubmed/35784862 http://dx.doi.org/10.3389/fphys.2022.915640 Text en Copyright © 2022 Michel, Messonnier, Giannesini, Chatel, Vilmen, Le Fur and Bendahan. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Physiology
Michel, Constance P.
Messonnier, Laurent A.
Giannesini, Benoit
Chatel, Benjamin
Vilmen, Christophe
Le Fur, Yann
Bendahan, David
Effects of Hydroxyurea on Skeletal Muscle Energetics and Function in a Mildly Anemic Mouse Model
title Effects of Hydroxyurea on Skeletal Muscle Energetics and Function in a Mildly Anemic Mouse Model
title_full Effects of Hydroxyurea on Skeletal Muscle Energetics and Function in a Mildly Anemic Mouse Model
title_fullStr Effects of Hydroxyurea on Skeletal Muscle Energetics and Function in a Mildly Anemic Mouse Model
title_full_unstemmed Effects of Hydroxyurea on Skeletal Muscle Energetics and Function in a Mildly Anemic Mouse Model
title_short Effects of Hydroxyurea on Skeletal Muscle Energetics and Function in a Mildly Anemic Mouse Model
title_sort effects of hydroxyurea on skeletal muscle energetics and function in a mildly anemic mouse model
topic Physiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9240423/
https://www.ncbi.nlm.nih.gov/pubmed/35784862
http://dx.doi.org/10.3389/fphys.2022.915640
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