A Leak-Free Inducible CRISPRi/a System for Gene Functional Studies in Plasmodium falciparum

By fusing catalytically dead Cas9 (dCas9) to active domains of histone deacetylase (Sir2a) or acetyltransferase (GCN5), this CRISPR interference/activation (CRISPRi/a) system allows gene regulation at the transcriptional level without causing permanent changes in the parasite genome. However, the co...

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Detalles Bibliográficos
Autores principales: Liang, Xiaoying, Boonhok, Rachasak, Siddiqui, Faiza Amber, Xiao, Bo, Li, Xiaolian, Qin, Junling, Min, Hui, Jiang, Lubin, Cui, Liwang, Miao, Jun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2022
Materias:
Resource Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9241666/
https://www.ncbi.nlm.nih.gov/pubmed/35510853
http://dx.doi.org/10.1128/spectrum.02782-21

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9241666/
https://www.ncbi.nlm.nih.gov/pubmed/35510853
http://dx.doi.org/10.1128/spectrum.02782-21

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