High-throughput sequencing SELEX for the determination of DNA-binding protein specificities in vitro

High-throughput sequencing SELEX (HT-SELEX) is a powerful technique for unbiased determination of preferred target motifs of DNA-binding proteins in vitro. The procedure depends upon selection of DNA binding sites from a random library of oligonucleotides by purifying protein-DNA complexes and ampli...

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Detalles Bibliográficos
Autores principales: Pantier, Raphaël, Chhatbar, Kashyap, Alston, Grace, Lee, Heng Yang, Bird, Adrian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9243297/
https://www.ncbi.nlm.nih.gov/pubmed/35776646
http://dx.doi.org/10.1016/j.xpro.2022.101490

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9243297/
https://www.ncbi.nlm.nih.gov/pubmed/35776646
http://dx.doi.org/10.1016/j.xpro.2022.101490

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