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High-throughput sequencing SELEX for the determination of DNA-binding protein specificities in vitro
High-throughput sequencing SELEX (HT-SELEX) is a powerful technique for unbiased determination of preferred target motifs of DNA-binding proteins in vitro. The procedure depends upon selection of DNA binding sites from a random library of oligonucleotides by purifying protein-DNA complexes and ampli...
| Autores principales: | , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2022
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9243297/ https://www.ncbi.nlm.nih.gov/pubmed/35776646 http://dx.doi.org/10.1016/j.xpro.2022.101490 |