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Long-term imaging of individual mRNA molecules in living cells

Single-cell imaging of individual mRNAs has revealed core mechanisms of the central dogma. However, most approaches require cell fixation or have limited sensitivity for live-cell applications. Here, we describe SunRISER (SunTag-based reporter for imaging signal-enriched mRNA), a computationally and...

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Detalles Bibliográficos
Autores principales: Guo, Yue, Lee, Robin E.C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9243547/
https://www.ncbi.nlm.nih.gov/pubmed/35784652
http://dx.doi.org/10.1016/j.crmeth.2022.100226