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Development of a fluorescent probe hydrolysis-insulated isothermal PCR for rapid and sensitive on-site detection of African swine fever virus
• A probe-based insulated isothermal PCR (iiPCR) assay was developed for rapid and onsite detection of ASFV. • The developed iiPCR showed similar sensitivity and specificity with OIE recommended real-time PCR. • Blood samples could be directly applied as PCR template in iiPCR without DNA extraction....
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Wuhan Institute of Virology, Chinese Academy of Sciences
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9243616/ https://www.ncbi.nlm.nih.gov/pubmed/35257964 http://dx.doi.org/10.1016/j.virs.2022.03.002 |