Development of a fluorescent probe hydrolysis-insulated isothermal PCR for rapid and sensitive on-site detection of African swine fever virus

• A probe-based insulated isothermal PCR (iiPCR) assay was developed for rapid and onsite detection of ASFV. • The developed iiPCR showed similar sensitivity and specificity with OIE recommended real-time PCR. • Blood samples could be directly applied as PCR template in iiPCR without DNA extraction....

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Detalles Bibliográficos
Autores principales: Zou, Tianli, Deng, Junhua, Li, Xiangdong, Zhang, Shiyin, Chen, Lingyan, Hao, Liying, Zhuang, Jinshan, Wang, Heng, Zhang, Guihong, Ge, Shengxiang, Tian, Kegong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Wuhan Institute of Virology, Chinese Academy of Sciences 2022
Materias:
Letter
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9243616/
https://www.ncbi.nlm.nih.gov/pubmed/35257964
http://dx.doi.org/10.1016/j.virs.2022.03.002
Descripción
Sumario:• A probe-based insulated isothermal PCR (iiPCR) assay was developed for rapid and onsite detection of ASFV. • The developed iiPCR showed similar sensitivity and specificity with OIE recommended real-time PCR. • Blood samples could be directly applied as PCR template in iiPCR without DNA extraction.

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