Development of a fluorescent probe hydrolysis-insulated isothermal PCR for rapid and sensitive on-site detection of African swine fever virus

• A probe-based insulated isothermal PCR (iiPCR) assay was developed for rapid and onsite detection of ASFV. • The developed iiPCR showed similar sensitivity and specificity with OIE recommended real-time PCR. • Blood samples could be directly applied as PCR template in iiPCR without DNA extraction....

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Autores principales: Zou, Tianli, Deng, Junhua, Li, Xiangdong, Zhang, Shiyin, Chen, Lingyan, Hao, Liying, Zhuang, Jinshan, Wang, Heng, Zhang, Guihong, Ge, Shengxiang, Tian, Kegong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Wuhan Institute of Virology, Chinese Academy of Sciences 2022
Materias:
Letter
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9243616/
https://www.ncbi.nlm.nih.gov/pubmed/35257964
http://dx.doi.org/10.1016/j.virs.2022.03.002
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author Zou, Tianli
Deng, Junhua
Li, Xiangdong
Zhang, Shiyin
Chen, Lingyan
Hao, Liying
Zhuang, Jinshan
Wang, Heng
Zhang, Guihong
Ge, Shengxiang
Tian, Kegong
author_facet Zou, Tianli
Deng, Junhua
Li, Xiangdong
Zhang, Shiyin
Chen, Lingyan
Hao, Liying
Zhuang, Jinshan
Wang, Heng
Zhang, Guihong
Ge, Shengxiang
Tian, Kegong
author_sort Zou, Tianli
collection PubMed
description • A probe-based insulated isothermal PCR (iiPCR) assay was developed for rapid and onsite detection of ASFV. • The developed iiPCR showed similar sensitivity and specificity with OIE recommended real-time PCR. • Blood samples could be directly applied as PCR template in iiPCR without DNA extraction.
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spelling pubmed-92436162022-07-01 Development of a fluorescent probe hydrolysis-insulated isothermal PCR for rapid and sensitive on-site detection of African swine fever virus Zou, Tianli Deng, Junhua Li, Xiangdong Zhang, Shiyin Chen, Lingyan Hao, Liying Zhuang, Jinshan Wang, Heng Zhang, Guihong Ge, Shengxiang Tian, Kegong Virol Sin Letter • A probe-based insulated isothermal PCR (iiPCR) assay was developed for rapid and onsite detection of ASFV. • The developed iiPCR showed similar sensitivity and specificity with OIE recommended real-time PCR. • Blood samples could be directly applied as PCR template in iiPCR without DNA extraction. Wuhan Institute of Virology, Chinese Academy of Sciences 2022-03-04 /pmc/articles/PMC9243616/ /pubmed/35257964 http://dx.doi.org/10.1016/j.virs.2022.03.002 Text en © 2022 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Letter
Zou, Tianli
Deng, Junhua
Li, Xiangdong
Zhang, Shiyin
Chen, Lingyan
Hao, Liying
Zhuang, Jinshan
Wang, Heng
Zhang, Guihong
Ge, Shengxiang
Tian, Kegong
Development of a fluorescent probe hydrolysis-insulated isothermal PCR for rapid and sensitive on-site detection of African swine fever virus
title Development of a fluorescent probe hydrolysis-insulated isothermal PCR for rapid and sensitive on-site detection of African swine fever virus
title_full Development of a fluorescent probe hydrolysis-insulated isothermal PCR for rapid and sensitive on-site detection of African swine fever virus
title_fullStr Development of a fluorescent probe hydrolysis-insulated isothermal PCR for rapid and sensitive on-site detection of African swine fever virus
title_full_unstemmed Development of a fluorescent probe hydrolysis-insulated isothermal PCR for rapid and sensitive on-site detection of African swine fever virus
title_short Development of a fluorescent probe hydrolysis-insulated isothermal PCR for rapid and sensitive on-site detection of African swine fever virus
title_sort development of a fluorescent probe hydrolysis-insulated isothermal pcr for rapid and sensitive on-site detection of african swine fever virus
topic Letter
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9243616/
https://www.ncbi.nlm.nih.gov/pubmed/35257964
http://dx.doi.org/10.1016/j.virs.2022.03.002
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