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Cell-Free Protein Synthesis by Diversifying Bacterial Transcription Machinery

We have evaluated several approaches to increase protein synthesis in a cell-free coupled bacterial transcription and translation system. A strong pargC promoter, originally isolated from a moderate thermophilic bacterium Geobacillus stearothermophilus, was used to improve the performance of a cell-...

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Detalles Bibliográficos
Autores principales: Snapyan, Marina, Robin, Sylvain, Yeretssian, Garabet, Lecocq, Michèle, Marc, Frédéric, Sakanyan, Vehary
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9245472/
https://www.ncbi.nlm.nih.gov/pubmed/35822798
http://dx.doi.org/10.3390/biotech10040024