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Cell-Free Protein Synthesis by Diversifying Bacterial Transcription Machinery
We have evaluated several approaches to increase protein synthesis in a cell-free coupled bacterial transcription and translation system. A strong pargC promoter, originally isolated from a moderate thermophilic bacterium Geobacillus stearothermophilus, was used to improve the performance of a cell-...
Autores principales: | Snapyan, Marina, Robin, Sylvain, Yeretssian, Garabet, Lecocq, Michèle, Marc, Frédéric, Sakanyan, Vehary |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9245472/ https://www.ncbi.nlm.nih.gov/pubmed/35822798 http://dx.doi.org/10.3390/biotech10040024 |
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