Photocontrolled crRNA activation enables robust CRISPR-Cas12a diagnostics

CRISPR diagnostics based on nucleic acid amplification faces barriers to its commercial use, such as contamination risks and insufficient sensitivity. Here, we propose a robust solution involving optochemical control of CRISPR RNA (crRNA) activation in CRISPR detection. Based on this strategy, recom...

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Detalles Bibliográficos
Autores principales: Hu, Menglu, Qiu, Zhiqiang, Bi, Zirong, Tian, Tian, Jiang, Yongzhong, Zhou, Xiaoming
Formato: Online Artículo Texto
Lenguaje:English
Publicado: National Academy of Sciences 2022
Materias:
Biological Sciences
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9245704/
https://www.ncbi.nlm.nih.gov/pubmed/35727982
http://dx.doi.org/10.1073/pnas.2202034119

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9245704/
https://www.ncbi.nlm.nih.gov/pubmed/35727982
http://dx.doi.org/10.1073/pnas.2202034119

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