Thicker Ice Improves the Integrity and Angular Distribution of CDC48A Hexamers on Cryo-EM Grids

Many cryogenic electron microscopy (cryo-EM) single particle analyses are constrained by the sample preparation step upon which aggregation, dissociation, and/or preferential orientation of particles can be introduced. Here, we report how we solved these problems in the case of CDC48A, a hexameric A...

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Detalles Bibliográficos
Autores principales: Huntington, Brandon, Zhao, Lingyun, Bron, Patrick, Shahul Hameed, Umar F., Arold, Stefan T., Qureshi, Bilal M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Molecular Biosciences
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9247313/
https://www.ncbi.nlm.nih.gov/pubmed/35782862
http://dx.doi.org/10.3389/fmolb.2022.890390

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9247313/
https://www.ncbi.nlm.nih.gov/pubmed/35782862
http://dx.doi.org/10.3389/fmolb.2022.890390

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