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Comparing methods for immobilizing HIV-1 SOSIPs in ELISAs that evaluate antibody binding

Enzyme-linked immunosorbent assays (ELISAs) are used to evaluate binding of broadly neutralizing antibodies (bNAbs) and polyclonal sera to native-like HIV-1 Env SOSIPs. Methods for immobilizing SOSIPs on plates differ, which can lead to variable or, in some cases, misleading results. Three methods u...

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Detalles Bibliográficos
Autores principales: Dam, Kim-Marie A., Mutia, Patricia S., Bjorkman, Pamela J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9247892/
https://www.ncbi.nlm.nih.gov/pubmed/35778473
http://dx.doi.org/10.1038/s41598-022-15506-x