Structural and biophysical studies of new l-asparaginase variants: lessons from random mutagenesis of the prototypic Escherichia coli Ntn-amidohydrolase

This work reports the results of random mutagenesis of the Escherichia coli class 2 l-asparaginase EcAIII belonging to the Ntn-hydrolase family. New variants of EcAIII were studied using structural, biophysical and bioinformatic methods. Activity tests revealed that the l-asparaginase activity is ab...

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Detalles Bibliográficos
Autores principales: Loch, Joanna I., Klonecka, Agnieszka, Kądziołka, Kinga, Bonarek, Piotr, Barciszewski, Jakub, Imiolczyk, Barbara, Brzezinski, Krzysztof, Gilski, Mirosław, Jaskolski, Mariusz
Formato: Online Artículo Texto
Lenguaje:English
Publicado: International Union of Crystallography 2022
Materias:
Research Papers
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9248843/
https://www.ncbi.nlm.nih.gov/pubmed/35775990
http://dx.doi.org/10.1107/S2059798322005691

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9248843/
https://www.ncbi.nlm.nih.gov/pubmed/35775990
http://dx.doi.org/10.1107/S2059798322005691

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