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The fluorescent protein iLOV as a reporter for screening of high‐yield production of antimicrobial peptides in Pichia pastoris

The methylotrophic yeast Pichia pastoris is commonly used for the production of recombinant proteins at scale. The identification of an optimally overexpressing strain following transformation can be time and reagent consuming. Fluorescent reporters like GFP have been used to assist identification o...

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Detalles Bibliográficos
Autores principales: Kjeldsen, Annemette, Kay, Jack E., Baxter, Scott, McColm, Stephen, Serrano‐Amatriain, Cristina, Parker, Scott, Robb, Ellis, Arnold, S. Alison, Gilmour, Craig, Raper, Anna, Robertson, Graeme, Fleming, Robert, Smith, Brian O., Fotheringham, Ian G., Christie, John M., Magneschi, Leonardo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9249318/
https://www.ncbi.nlm.nih.gov/pubmed/35312165
http://dx.doi.org/10.1111/1751-7915.14034