A protocol to induce systemic autophagy and increase energy metabolism in mice using PEGylated arginine deiminase

Obesity is a prevalent metabolic disorder worldwide. Here, we describe a comprehensive protocol using pegylated arginine deiminase (ADI-EPG 20) to apply the concept that arginine depletion induces systemic autophagy to drive whole-body energy metabolism and weight loss in mice. We detail the steps f...

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Detalles Bibliográficos
Autores principales: Zhang, Yiming, DeBosch, Brian J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9249829/
https://www.ncbi.nlm.nih.gov/pubmed/35776644
http://dx.doi.org/10.1016/j.xpro.2022.101489
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author Zhang, Yiming
DeBosch, Brian J.
author_facet Zhang, Yiming
DeBosch, Brian J.
author_sort Zhang, Yiming
collection PubMed
description Obesity is a prevalent metabolic disorder worldwide. Here, we describe a comprehensive protocol using pegylated arginine deiminase (ADI-EPG 20) to apply the concept that arginine depletion induces systemic autophagy to drive whole-body energy metabolism and weight loss in mice. We detail the steps for cohort setup, mouse husbandry, and treatment and provide expected results under these conditions. For complete details on the use and execution of this protocol, please refer to Zhang et al. (2022a, 2022b).
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spelling pubmed-92498292022-07-03 A protocol to induce systemic autophagy and increase energy metabolism in mice using PEGylated arginine deiminase Zhang, Yiming DeBosch, Brian J. STAR Protoc Protocol Obesity is a prevalent metabolic disorder worldwide. Here, we describe a comprehensive protocol using pegylated arginine deiminase (ADI-EPG 20) to apply the concept that arginine depletion induces systemic autophagy to drive whole-body energy metabolism and weight loss in mice. We detail the steps for cohort setup, mouse husbandry, and treatment and provide expected results under these conditions. For complete details on the use and execution of this protocol, please refer to Zhang et al. (2022a, 2022b). Elsevier 2022-06-23 /pmc/articles/PMC9249829/ /pubmed/35776644 http://dx.doi.org/10.1016/j.xpro.2022.101489 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Zhang, Yiming
DeBosch, Brian J.
A protocol to induce systemic autophagy and increase energy metabolism in mice using PEGylated arginine deiminase
title A protocol to induce systemic autophagy and increase energy metabolism in mice using PEGylated arginine deiminase
title_full A protocol to induce systemic autophagy and increase energy metabolism in mice using PEGylated arginine deiminase
title_fullStr A protocol to induce systemic autophagy and increase energy metabolism in mice using PEGylated arginine deiminase
title_full_unstemmed A protocol to induce systemic autophagy and increase energy metabolism in mice using PEGylated arginine deiminase
title_short A protocol to induce systemic autophagy and increase energy metabolism in mice using PEGylated arginine deiminase
title_sort protocol to induce systemic autophagy and increase energy metabolism in mice using pegylated arginine deiminase
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9249829/
https://www.ncbi.nlm.nih.gov/pubmed/35776644
http://dx.doi.org/10.1016/j.xpro.2022.101489
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