Expansion microscopy of mouse brain organotypic slice cultures to study protein distribution

Assessing protein distribution with super-resolution in tissue is often complicated and restrictive. Here, we describe a protocol for immunostaining and expansion microscopy imaging of mouse brain organotypic slice cultures. We detail an Imaris analysis workflow to analyze the surface vs intracellul...

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Detalles Bibliográficos
Autores principales: Bissen, Diane, Kracht, Maximilian Ken, Foss, Franziska, Acker-Palmer, Amparo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9249947/
https://www.ncbi.nlm.nih.gov/pubmed/35776645
http://dx.doi.org/10.1016/j.xpro.2022.101507

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9249947/
https://www.ncbi.nlm.nih.gov/pubmed/35776645
http://dx.doi.org/10.1016/j.xpro.2022.101507

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