Protocol for determining the average speed and frequency of kinesin and dynein-driven intraflagellar transport (IFT) in C. elegans

Here, we present a protocol to image a fluorescent-labeled intraflagellar transport (IFT) component in Caenorhabditis elegans with fluorescence microscopy, including steps of sample preparations, in vivo live-cell imaging, and post-microscopy analysis with kymographs. This protocol breaks down all p...

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Detalles Bibliográficos
Autores principales: Turan, Merve G., Kantarci, Hanife, Temtek, Sadiye D., Cakici, Onur, Cevik, Sebiha, Kaplan, Oktay I.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9250072/
https://www.ncbi.nlm.nih.gov/pubmed/35776634
http://dx.doi.org/10.1016/j.xpro.2022.101498

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9250072/
https://www.ncbi.nlm.nih.gov/pubmed/35776634
http://dx.doi.org/10.1016/j.xpro.2022.101498

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