Protocol for determining the average speed and frequency of kinesin and dynein-driven intraflagellar transport (IFT) in C. elegans

Here, we present a protocol to image a fluorescent-labeled intraflagellar transport (IFT) component in Caenorhabditis elegans with fluorescence microscopy, including steps of sample preparations, in vivo live-cell imaging, and post-microscopy analysis with kymographs. This protocol breaks down all p...

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Detalles Bibliográficos
Autores principales: Turan, Merve G., Kantarci, Hanife, Temtek, Sadiye D., Cakici, Onur, Cevik, Sebiha, Kaplan, Oktay I.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9250072/
https://www.ncbi.nlm.nih.gov/pubmed/35776634
http://dx.doi.org/10.1016/j.xpro.2022.101498
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author Turan, Merve G.
Kantarci, Hanife
Temtek, Sadiye D.
Cakici, Onur
Cevik, Sebiha
Kaplan, Oktay I.
author_facet Turan, Merve G.
Kantarci, Hanife
Temtek, Sadiye D.
Cakici, Onur
Cevik, Sebiha
Kaplan, Oktay I.
author_sort Turan, Merve G.
collection PubMed
description Here, we present a protocol to image a fluorescent-labeled intraflagellar transport (IFT) component in Caenorhabditis elegans with fluorescence microscopy, including steps of sample preparations, in vivo live-cell imaging, and post-microscopy analysis with kymographs. This protocol breaks down all processes into three categories: (1) pre-imaging preparations, (2) preparations for the time of image acquisition, and (3) post-imaging analyses. The protocol can be applied to determine the speed and frequency of moving particles. For complete details on the use and execution of this protocol, please refer to Cevik et al. (2021).
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spelling pubmed-92500722022-07-03 Protocol for determining the average speed and frequency of kinesin and dynein-driven intraflagellar transport (IFT) in C. elegans Turan, Merve G. Kantarci, Hanife Temtek, Sadiye D. Cakici, Onur Cevik, Sebiha Kaplan, Oktay I. STAR Protoc Protocol Here, we present a protocol to image a fluorescent-labeled intraflagellar transport (IFT) component in Caenorhabditis elegans with fluorescence microscopy, including steps of sample preparations, in vivo live-cell imaging, and post-microscopy analysis with kymographs. This protocol breaks down all processes into three categories: (1) pre-imaging preparations, (2) preparations for the time of image acquisition, and (3) post-imaging analyses. The protocol can be applied to determine the speed and frequency of moving particles. For complete details on the use and execution of this protocol, please refer to Cevik et al. (2021). Elsevier 2022-06-24 /pmc/articles/PMC9250072/ /pubmed/35776634 http://dx.doi.org/10.1016/j.xpro.2022.101498 Text en © 2022 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Turan, Merve G.
Kantarci, Hanife
Temtek, Sadiye D.
Cakici, Onur
Cevik, Sebiha
Kaplan, Oktay I.
Protocol for determining the average speed and frequency of kinesin and dynein-driven intraflagellar transport (IFT) in C. elegans
title Protocol for determining the average speed and frequency of kinesin and dynein-driven intraflagellar transport (IFT) in C. elegans
title_full Protocol for determining the average speed and frequency of kinesin and dynein-driven intraflagellar transport (IFT) in C. elegans
title_fullStr Protocol for determining the average speed and frequency of kinesin and dynein-driven intraflagellar transport (IFT) in C. elegans
title_full_unstemmed Protocol for determining the average speed and frequency of kinesin and dynein-driven intraflagellar transport (IFT) in C. elegans
title_short Protocol for determining the average speed and frequency of kinesin and dynein-driven intraflagellar transport (IFT) in C. elegans
title_sort protocol for determining the average speed and frequency of kinesin and dynein-driven intraflagellar transport (ift) in c. elegans
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9250072/
https://www.ncbi.nlm.nih.gov/pubmed/35776634
http://dx.doi.org/10.1016/j.xpro.2022.101498
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