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Temperature-responsive optogenetic probes of cell signaling
We describe single-component optogenetic probes whose activation dynamics depend on both light and temperature. We used the BcLOV4 photoreceptor to stimulate Ras and PI3K signaling in mammalian cells, allowing activation over a large dynamic range with low basal levels. Surprisingly, we found that B...
Autores principales: | Benman, William, Berlew, Erin E., Deng, Hao, Parker, Caitlyn, Kuznetsov, Ivan A., Lim, Bomyi, Siekmann, Arndt F., Chow, Brian Y., Bugaj, Lukasz J. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9252025/ https://www.ncbi.nlm.nih.gov/pubmed/34937907 http://dx.doi.org/10.1038/s41589-021-00917-0 |
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