Engineering of ultraID, a compact and hyperactive enzyme for proximity-dependent biotinylation in living cells

Proximity-dependent biotinylation (PDB) combined with mass spectrometry analysis has established itself as a key technology to study protein-protein interactions in living cells. A widespread approach, BioID, uses an abortive variant of the E. coli BirA biotin protein ligase, a quite bulky enzyme wi...

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Detalles Bibliográficos
Autores principales: Kubitz, Lea, Bitsch, Sebastian, Zhao, Xiyan, Schmitt, Kerstin, Deweid, Lukas, Roehrig, Amélie, Barazzone, Elisa Cappio, Valerius, Oliver, Kolmar, Harald, Béthune, Julien
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9253107/
https://www.ncbi.nlm.nih.gov/pubmed/35788163
http://dx.doi.org/10.1038/s42003-022-03604-5

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9253107/
https://www.ncbi.nlm.nih.gov/pubmed/35788163
http://dx.doi.org/10.1038/s42003-022-03604-5

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