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Engineering of ultraID, a compact and hyperactive enzyme for proximity-dependent biotinylation in living cells
Proximity-dependent biotinylation (PDB) combined with mass spectrometry analysis has established itself as a key technology to study protein-protein interactions in living cells. A widespread approach, BioID, uses an abortive variant of the E. coli BirA biotin protein ligase, a quite bulky enzyme wi...
| Autores principales: | , , , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Nature Publishing Group UK
2022
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9253107/ https://www.ncbi.nlm.nih.gov/pubmed/35788163 http://dx.doi.org/10.1038/s42003-022-03604-5 |