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Protocols for measuring cold-evoked neural activity and cold tolerance in Drosophila larvae following fictive cold acclimation

Here, we outline protocols to study cold acclimation in Drosophila from a neurobiological perspective, starting with fictive cold acclimation using a custom-built optogenetics-housing apparatus we call the OptoBox. We also provide detailed steps for single-unit electrophysiological recordings from l...

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Detalles Bibliográficos
Autores principales: Himmel, Nathaniel J., Sakurai, Akira, Donaldson, Kevin J., Cox, Daniel N.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9253850/
https://www.ncbi.nlm.nih.gov/pubmed/35776643
http://dx.doi.org/10.1016/j.xpro.2022.101510