Protocol to analyze transmigration of human cytotoxic T cells under physiological flow conditions in vitro

This protocol presents an assay for transmigration analysis of human cytotoxic T cells (CTL) under physiological flow in vitro. We describe detailed analysis steps of human CTL behavior, from adhesion to diapedesis, using live cell imaging which cannot be achieved by in vivo imaging. The flow system...

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Detalles Bibliográficos
Autores principales: Schoppmeyer, Rouven, van Buul, Jaap D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9254146/
https://www.ncbi.nlm.nih.gov/pubmed/35776649
http://dx.doi.org/10.1016/j.xpro.2022.101509

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9254146/
https://www.ncbi.nlm.nih.gov/pubmed/35776649
http://dx.doi.org/10.1016/j.xpro.2022.101509

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