Protocol to analyze transmigration of human cytotoxic T cells under physiological flow conditions in vitro

This protocol presents an assay for transmigration analysis of human cytotoxic T cells (CTL) under physiological flow in vitro. We describe detailed analysis steps of human CTL behavior, from adhesion to diapedesis, using live cell imaging which cannot be achieved by in vivo imaging. The flow system...

Descripción completa

Detalles Bibliográficos
Autores principales: Schoppmeyer, Rouven, van Buul, Jaap D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9254146/
https://www.ncbi.nlm.nih.gov/pubmed/35776649
http://dx.doi.org/10.1016/j.xpro.2022.101509
_version_ 1784740631415881728
author Schoppmeyer, Rouven
van Buul, Jaap D.
author_facet Schoppmeyer, Rouven
van Buul, Jaap D.
author_sort Schoppmeyer, Rouven
collection PubMed
description This protocol presents an assay for transmigration analysis of human cytotoxic T cells (CTL) under physiological flow in vitro. We describe detailed analysis steps of human CTL behavior, from adhesion to diapedesis, using live cell imaging which cannot be achieved by in vivo imaging. The flow system is made of 2D plastic surfaces covered by an endothelial monolayer limiting the system but allows for quantitative analysis of CTL behavior with high modifiability. For complete details on the use and execution of this protocol, please refer to Schoppmeyer et al. (2022).
format Online
Article
Text
id pubmed-9254146
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Elsevier
record_format MEDLINE/PubMed
spelling pubmed-92541462022-07-06 Protocol to analyze transmigration of human cytotoxic T cells under physiological flow conditions in vitro Schoppmeyer, Rouven van Buul, Jaap D. STAR Protoc Protocol This protocol presents an assay for transmigration analysis of human cytotoxic T cells (CTL) under physiological flow in vitro. We describe detailed analysis steps of human CTL behavior, from adhesion to diapedesis, using live cell imaging which cannot be achieved by in vivo imaging. The flow system is made of 2D plastic surfaces covered by an endothelial monolayer limiting the system but allows for quantitative analysis of CTL behavior with high modifiability. For complete details on the use and execution of this protocol, please refer to Schoppmeyer et al. (2022). Elsevier 2022-06-30 /pmc/articles/PMC9254146/ /pubmed/35776649 http://dx.doi.org/10.1016/j.xpro.2022.101509 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Protocol
Schoppmeyer, Rouven
van Buul, Jaap D.
Protocol to analyze transmigration of human cytotoxic T cells under physiological flow conditions in vitro
title Protocol to analyze transmigration of human cytotoxic T cells under physiological flow conditions in vitro
title_full Protocol to analyze transmigration of human cytotoxic T cells under physiological flow conditions in vitro
title_fullStr Protocol to analyze transmigration of human cytotoxic T cells under physiological flow conditions in vitro
title_full_unstemmed Protocol to analyze transmigration of human cytotoxic T cells under physiological flow conditions in vitro
title_short Protocol to analyze transmigration of human cytotoxic T cells under physiological flow conditions in vitro
title_sort protocol to analyze transmigration of human cytotoxic t cells under physiological flow conditions in vitro
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9254146/
https://www.ncbi.nlm.nih.gov/pubmed/35776649
http://dx.doi.org/10.1016/j.xpro.2022.101509
work_keys_str_mv AT schoppmeyerrouven protocoltoanalyzetransmigrationofhumancytotoxictcellsunderphysiologicalflowconditionsinvitro
AT vanbuuljaapd protocoltoanalyzetransmigrationofhumancytotoxictcellsunderphysiologicalflowconditionsinvitro

Ejemplares similares