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Protocol to analyze transmigration of human cytotoxic T cells under physiological flow conditions in vitro
This protocol presents an assay for transmigration analysis of human cytotoxic T cells (CTL) under physiological flow in vitro. We describe detailed analysis steps of human CTL behavior, from adhesion to diapedesis, using live cell imaging which cannot be achieved by in vivo imaging. The flow system...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2022
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9254146/ https://www.ncbi.nlm.nih.gov/pubmed/35776649 http://dx.doi.org/10.1016/j.xpro.2022.101509 |