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Generation of hematopoietic lineage cell-specific chimeric mice using retrovirus-transduced fetal liver cells

Hematopoietic lineage cell-specific transgenic or knockout mice provide a valuable platform to identify the role of specific genes in hematopoiesis in vivo. Here, we describe protocols for preparation of retroviruses for overexpression or knockdown of a gene of interest, retroviral transduction of f...

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Detalles Bibliográficos
Autores principales: Hong, Hyebeen, Lee, Yoontae
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9256946/
https://www.ncbi.nlm.nih.gov/pubmed/35779265
http://dx.doi.org/10.1016/j.xpro.2022.101526