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Generation of hematopoietic lineage cell-specific chimeric mice using retrovirus-transduced fetal liver cells
Hematopoietic lineage cell-specific transgenic or knockout mice provide a valuable platform to identify the role of specific genes in hematopoiesis in vivo. Here, we describe protocols for preparation of retroviruses for overexpression or knockdown of a gene of interest, retroviral transduction of f...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2022
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9256946/ https://www.ncbi.nlm.nih.gov/pubmed/35779265 http://dx.doi.org/10.1016/j.xpro.2022.101526 |