Cargando…

Coexpression of MEIOTIC-TOPOISOMERASE VIB-dCas9 with guide RNAs specific to a recombination hotspot is insufficient to increase crossover frequency in Arabidopsis

During meiosis, homologous chromosomes pair and recombine, which can result in reciprocal crossovers that increase genetic diversity. Crossovers are unevenly distributed along eukaryote chromosomes and show repression in heterochromatin and the centromeres. Within the chromosome arms, crossovers are...

Descripción completa

Detalles Bibliográficos
Autores principales: Yelina, Nataliya E, Holland, Daniel, Gonzalez-Jorge, Sabrina, Hirsz, Dominique, Yang, Ziyi, Henderson, Ian R
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9258527/
https://www.ncbi.nlm.nih.gov/pubmed/35485960
http://dx.doi.org/10.1093/g3journal/jkac105
_version_ 1784741573474385920
author Yelina, Nataliya E
Holland, Daniel
Gonzalez-Jorge, Sabrina
Hirsz, Dominique
Yang, Ziyi
Henderson, Ian R
author_facet Yelina, Nataliya E
Holland, Daniel
Gonzalez-Jorge, Sabrina
Hirsz, Dominique
Yang, Ziyi
Henderson, Ian R
author_sort Yelina, Nataliya E
collection PubMed
description During meiosis, homologous chromosomes pair and recombine, which can result in reciprocal crossovers that increase genetic diversity. Crossovers are unevenly distributed along eukaryote chromosomes and show repression in heterochromatin and the centromeres. Within the chromosome arms, crossovers are often concentrated in hotspots, which are typically in the kilobase range. The uneven distribution of crossovers along chromosomes, together with their low number per meiosis, creates a limitation during crop breeding, where recombination can be beneficial. Therefore, targeting crossovers to specific genome locations has the potential to accelerate crop improvement. In plants, meiotic crossovers are initiated by DNA double-strand breaks that are catalyzed by SPO11 complexes, which consist of 2 catalytic (SPO11-1 and SPO11-2) and 2 noncatalytic subunits (MTOPVIB). We used the model plant Arabidopsis thaliana to coexpress an MTOPVIB-dCas9 fusion protein with guide RNAs specific to the 3a crossover hotspot. We observed that this was insufficient to significantly change meiotic crossover frequency or pattern within 3a. We discuss the implications of our findings for targeting meiotic recombination within plant genomes.
format Online
Article
Text
id pubmed-9258527
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Oxford University Press
record_format MEDLINE/PubMed
spelling pubmed-92585272022-07-07 Coexpression of MEIOTIC-TOPOISOMERASE VIB-dCas9 with guide RNAs specific to a recombination hotspot is insufficient to increase crossover frequency in Arabidopsis Yelina, Nataliya E Holland, Daniel Gonzalez-Jorge, Sabrina Hirsz, Dominique Yang, Ziyi Henderson, Ian R G3 (Bethesda) Investigation During meiosis, homologous chromosomes pair and recombine, which can result in reciprocal crossovers that increase genetic diversity. Crossovers are unevenly distributed along eukaryote chromosomes and show repression in heterochromatin and the centromeres. Within the chromosome arms, crossovers are often concentrated in hotspots, which are typically in the kilobase range. The uneven distribution of crossovers along chromosomes, together with their low number per meiosis, creates a limitation during crop breeding, where recombination can be beneficial. Therefore, targeting crossovers to specific genome locations has the potential to accelerate crop improvement. In plants, meiotic crossovers are initiated by DNA double-strand breaks that are catalyzed by SPO11 complexes, which consist of 2 catalytic (SPO11-1 and SPO11-2) and 2 noncatalytic subunits (MTOPVIB). We used the model plant Arabidopsis thaliana to coexpress an MTOPVIB-dCas9 fusion protein with guide RNAs specific to the 3a crossover hotspot. We observed that this was insufficient to significantly change meiotic crossover frequency or pattern within 3a. We discuss the implications of our findings for targeting meiotic recombination within plant genomes. Oxford University Press 2022-04-29 /pmc/articles/PMC9258527/ /pubmed/35485960 http://dx.doi.org/10.1093/g3journal/jkac105 Text en © The Author(s) 2022. Published by Oxford University Press on behalf of Genetics Society of America. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Investigation
Yelina, Nataliya E
Holland, Daniel
Gonzalez-Jorge, Sabrina
Hirsz, Dominique
Yang, Ziyi
Henderson, Ian R
Coexpression of MEIOTIC-TOPOISOMERASE VIB-dCas9 with guide RNAs specific to a recombination hotspot is insufficient to increase crossover frequency in Arabidopsis
title Coexpression of MEIOTIC-TOPOISOMERASE VIB-dCas9 with guide RNAs specific to a recombination hotspot is insufficient to increase crossover frequency in Arabidopsis
title_full Coexpression of MEIOTIC-TOPOISOMERASE VIB-dCas9 with guide RNAs specific to a recombination hotspot is insufficient to increase crossover frequency in Arabidopsis
title_fullStr Coexpression of MEIOTIC-TOPOISOMERASE VIB-dCas9 with guide RNAs specific to a recombination hotspot is insufficient to increase crossover frequency in Arabidopsis
title_full_unstemmed Coexpression of MEIOTIC-TOPOISOMERASE VIB-dCas9 with guide RNAs specific to a recombination hotspot is insufficient to increase crossover frequency in Arabidopsis
title_short Coexpression of MEIOTIC-TOPOISOMERASE VIB-dCas9 with guide RNAs specific to a recombination hotspot is insufficient to increase crossover frequency in Arabidopsis
title_sort coexpression of meiotic-topoisomerase vib-dcas9 with guide rnas specific to a recombination hotspot is insufficient to increase crossover frequency in arabidopsis
topic Investigation
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9258527/
https://www.ncbi.nlm.nih.gov/pubmed/35485960
http://dx.doi.org/10.1093/g3journal/jkac105
work_keys_str_mv AT yelinanataliyae coexpressionofmeiotictopoisomerasevibdcas9withguidernasspecifictoarecombinationhotspotisinsufficienttoincreasecrossoverfrequencyinarabidopsis
AT hollanddaniel coexpressionofmeiotictopoisomerasevibdcas9withguidernasspecifictoarecombinationhotspotisinsufficienttoincreasecrossoverfrequencyinarabidopsis
AT gonzalezjorgesabrina coexpressionofmeiotictopoisomerasevibdcas9withguidernasspecifictoarecombinationhotspotisinsufficienttoincreasecrossoverfrequencyinarabidopsis
AT hirszdominique coexpressionofmeiotictopoisomerasevibdcas9withguidernasspecifictoarecombinationhotspotisinsufficienttoincreasecrossoverfrequencyinarabidopsis
AT yangziyi coexpressionofmeiotictopoisomerasevibdcas9withguidernasspecifictoarecombinationhotspotisinsufficienttoincreasecrossoverfrequencyinarabidopsis
AT hendersonianr coexpressionofmeiotictopoisomerasevibdcas9withguidernasspecifictoarecombinationhotspotisinsufficienttoincreasecrossoverfrequencyinarabidopsis