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Accelerated strain construction and characterization of C. glutamicum protein secretion by laboratory automation

ABSTRACT: Secretion of bacterial proteins into the culture medium simplifies downstream processing by avoiding cell disruption for target protein purification. However, a suitable signal peptide for efficient secretion needs to be identified, and currently, there are no tools available to predict op...

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Detalles Bibliográficos
Autores principales: Müller, Carolin, Bakkes, Patrick J., Lenz, Patrick, Waffenschmidt, Vera, Helleckes, Laura M., Jaeger, Karl-Erich, Wiechert, Wolfgang, Knapp, Andreas, Freudl, Roland, Oldiges, Marco
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9259529/
https://www.ncbi.nlm.nih.gov/pubmed/35759036
http://dx.doi.org/10.1007/s00253-022-12017-7