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Accelerated strain construction and characterization of C. glutamicum protein secretion by laboratory automation
ABSTRACT: Secretion of bacterial proteins into the culture medium simplifies downstream processing by avoiding cell disruption for target protein purification. However, a suitable signal peptide for efficient secretion needs to be identified, and currently, there are no tools available to predict op...
Autores principales: | Müller, Carolin, Bakkes, Patrick J., Lenz, Patrick, Waffenschmidt, Vera, Helleckes, Laura M., Jaeger, Karl-Erich, Wiechert, Wolfgang, Knapp, Andreas, Freudl, Roland, Oldiges, Marco |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Berlin Heidelberg
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9259529/ https://www.ncbi.nlm.nih.gov/pubmed/35759036 http://dx.doi.org/10.1007/s00253-022-12017-7 |
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