Cargando…

Biophysical Screening Pipeline for Cryo-EM Grid Preparation of Membrane Proteins

Successful sample preparation is the foundation to any structural biology technique. Membrane proteins are of particular interest as these are important targets for drug design, but also notoriously difficult to work with. For electron cryo-microscopy (cryo-EM), the biophysical characterization of s...

Descripción completa

Detalles Bibliográficos
Autores principales:	Niebling, Stephan, Veith, Katharina, Vollmer, Benjamin, Lizarrondo, Javier, Burastero, Osvaldo, Schiller, Janina, Struve García, Angelica, Lewe, Philipp, Seuring, Carolin, Witt, Susanne, García-Alai, María
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Frontiers Media S.A. 2022
Materias:	Molecular Biosciences
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9259969/ https://www.ncbi.nlm.nih.gov/pubmed/35813810 http://dx.doi.org/10.3389/fmolb.2022.882288

Ejemplares similares

eSPC: an online data-analysis platform for molecular biophysics
por: Burastero, Osvaldo, et al.
Publicado: (2021)

The Cryo-EM structures of two amphibian antimicrobial cross-β amyloid fibrils
por: Bücker, Robert, et al.
Publicado: (2022)

Cryo-EM grid optimization for membrane proteins
por: Kampjut, Domen, et al.
Publicado: (2021)

On-grid and in-flow mixing for time-resolved cryo-EM
por: Klebl, David P., et al.
Publicado: (2021)

Towards sub-millisecond cryo-EM grid preparation
por: Klebl, David P., et al.
Publicado: (2022)

Light ‘Em up: Efficient Screening of Gold Foil Grids in Cryo-EM
por: Hagen, Wim J. H.
Publicado: (2022)

Ice thickness monitoring for cryo-EM grids by interferometry imaging
por: Hohle, Markus Matthias, et al.
Publicado: (2022)

Routine single particle CryoEM sample and grid characterization by tomography
por: Noble, Alex J, et al.
Publicado: (2018)

Functionalized graphene grids with various charges for single-particle cryo-EM
por: Lu, Ye, et al.
Publicado: (2022)

Batch Production of High-Quality Graphene Grids for Cryo-EM: Cryo-EM Structure of Methylococcus capsulatus Soluble Methane Monooxygenase Hydroxylase
por: Ahn, Eungjin, et al.
Publicado: (2023)

A microtubule RELION-based pipeline for cryo-EM image processing
por: Cook, Alexander D., et al.
Publicado: (2020)

Cryo-EM Grid Preparation of Membrane Protein Samples for Single Particle Analysis
por: Sgro, Germán G., et al.
Publicado: (2018)

Sample deposition onto cryo-EM grids: from sprays to jets and back
por: Klebl, David P., et al.
Publicado: (2020)

A cryo-EM grid preparation device for time-resolved structural studies
por: Kontziampasis, Dimitrios, et al.
Publicado: (2019)

Fully automated multi-grid cryoEM screening using Smart Leginon
por: Cheng, Anchi, et al.
Publicado: (2023)

It started with a Cys: Spontaneous cysteine modification during cryo-EM grid preparation
por: Klebl, David P., et al.
Publicado: (2022)

Epoxidized graphene grid for highly efficient high-resolution cryoEM structural analysis
por: Fujita, Junso, et al.
Publicado: (2023)

Polyelectrolyte coating of cryo-EM grids improves lateral distribution and prevents aggregation of macromolecules
por: Hrebík, Dominik, et al.
Publicado: (2022)

FoldAffinity: binding affinities from nDSF experiments
por: Niebling, Stephan, et al.
Publicado: (2021)

General and robust covalently linked graphene oxide affinity grids for high-resolution cryo-EM
por: Wang, Feng, et al.
Publicado: (2020)

Need for Speed: Examining Protein Behavior during CryoEM Grid Preparation at Different Timescales
por: Klebl, David P., et al.
Publicado: (2020)

Structure of the endocytic adaptor complex reveals the basis for efficient membrane anchoring during clathrin-mediated endocytosis
por: Lizarrondo, Javier, et al.
Publicado: (2021)

Thicker Ice Improves the Integrity and Angular Distribution of CDC48A Hexamers on Cryo-EM Grids
por: Huntington, Brandon, et al.
Publicado: (2022)

Raynals, an online tool for the analysis of dynamic light scattering
por: Burastero, Osvaldo, et al.
Publicado: (2023)

A snapshot of cryo‐EM
por: Skiniotis, Georgios
Publicado: (2016)

Interpreting the cryo-EM map
por: Rosenthal, Peter B.
Publicado: (2019)

COVID-19 and cryo-EM
por: Subramaniam, Sriram
Publicado: (2020)

Cryo-EM at ACA 2022
por: Subramaniam, Sriram, et al.
Publicado: (2022)

Tools for the cryo-EM gold rush: going from the cryo-EM map to the atomistic model
por: Kim, Doo Nam, et al.
Publicado: (2017)

Exploring applications of crowdsourcing to cryo-EM
por: Bruggemann, Jacob, et al.
Publicado: (2018)

Cryo-EM Structure of the Exocyst Complex
por: Mei, Kunrong, et al.
Publicado: (2018)

Density modification of cryo-EM maps
por: Terwilliger, Thomas C., et al.
Publicado: (2020)

Fast multiscale reconstruction for Cryo-EM()
por: Donati, Laurène, et al.
Publicado: (2018)

Cryo‐EM targets in CASP14
por: Cragnolini, Tristan, et al.
Publicado: (2021)

Cryo-EM structure of the SEA complex
por: Tafur, Lucas, et al.
Publicado: (2022)

Cryo‐EM structures of coagulation factors
por: Di Cera, Enrico, et al.
Publicado: (2022)

Structure determination of GPCRs: cryo-EM compared with X-ray crystallography
por: García-Nafría, Javier, et al.
Publicado: (2021)

AlphaFold2 and CryoEM: Revisiting CryoEM modeling in near-atomic resolution density maps
por: Hryc, Corey F., et al.
Publicado: (2022)

Detergents and alternatives in cryo-EM studies of membrane proteins: Detergents and alternatives in cryo-EM studies of membrane proteins
por: Li, Shuo
Publicado: (2022)

‘Cryo-EM’: electron cryomicroscopy, cryo electron microscopy or something else?
por: Henderson, Richard, et al.
Publicado: (2023)

Cannot write session to /tmp/vufind_sessions/sess_db1so1i5fr201c79e8hg5poe5u