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The Effect of Triptolide Combined With Crocin on Arthritis in Mice: From Side Effect Attenuation to Therapy

Clinical use of triptolide (TP) is restricted due to severe toxicity. This study assessed the protective effect of crocin (CR) as a natural antioxidant against TP-induced toxicity in bovine collagen type II-induced arthritis (CIA) in mice. The mice in the CIA model group showed macroscopic signs of...

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Autores principales: Yan, Min, Yan, Yinyin, Zhang, Zhenqiang, Wang, Guoqiang, Shi, Wenbo, Jiang, Mengyuan, Zhao, Junwei, Wu, Xiangxiang, Zeng, Huahui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9260116/
https://www.ncbi.nlm.nih.gov/pubmed/35814255
http://dx.doi.org/10.3389/fphar.2022.908227
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author Yan, Min
Yan, Yinyin
Zhang, Zhenqiang
Wang, Guoqiang
Shi, Wenbo
Jiang, Mengyuan
Zhao, Junwei
Wu, Xiangxiang
Zeng, Huahui
author_facet Yan, Min
Yan, Yinyin
Zhang, Zhenqiang
Wang, Guoqiang
Shi, Wenbo
Jiang, Mengyuan
Zhao, Junwei
Wu, Xiangxiang
Zeng, Huahui
author_sort Yan, Min
collection PubMed
description Clinical use of triptolide (TP) is restricted due to severe toxicity. This study assessed the protective effect of crocin (CR) as a natural antioxidant against TP-induced toxicity in bovine collagen type II-induced arthritis (CIA) in mice. The mice in the CIA model group showed macroscopic signs of severe arthritis. The anti-arthritis effects in the control, TP + CR, and TP groups were evaluated through assessment of foot volume, arthritis score, and proinflammatory cytokines, and collagen antibody assay. Crocin reduced TP-induced toxicity, as evidenced by evaluation of survival rate, body weight, visceral index, hepatic and renal functions, histopathologic analyses, and antioxidant enzyme activities. Transcriptome sequencing resulted in identification of 76 differentially expressed genes (DEGs) associated with hepatotoxicity between the TP and TP + CR groups. Of these, Three DEGs (Cyp1a2,Gsta4, and Gstp1) were validated using quantitative real-time PCR analysis. In conclusion, CR protected CIA mice from TP-induced toxicity through modulation of the cytochrome P450 and glutathione metabolism pathways.
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spelling pubmed-92601162022-07-08 The Effect of Triptolide Combined With Crocin on Arthritis in Mice: From Side Effect Attenuation to Therapy Yan, Min Yan, Yinyin Zhang, Zhenqiang Wang, Guoqiang Shi, Wenbo Jiang, Mengyuan Zhao, Junwei Wu, Xiangxiang Zeng, Huahui Front Pharmacol Pharmacology Clinical use of triptolide (TP) is restricted due to severe toxicity. This study assessed the protective effect of crocin (CR) as a natural antioxidant against TP-induced toxicity in bovine collagen type II-induced arthritis (CIA) in mice. The mice in the CIA model group showed macroscopic signs of severe arthritis. The anti-arthritis effects in the control, TP + CR, and TP groups were evaluated through assessment of foot volume, arthritis score, and proinflammatory cytokines, and collagen antibody assay. Crocin reduced TP-induced toxicity, as evidenced by evaluation of survival rate, body weight, visceral index, hepatic and renal functions, histopathologic analyses, and antioxidant enzyme activities. Transcriptome sequencing resulted in identification of 76 differentially expressed genes (DEGs) associated with hepatotoxicity between the TP and TP + CR groups. Of these, Three DEGs (Cyp1a2,Gsta4, and Gstp1) were validated using quantitative real-time PCR analysis. In conclusion, CR protected CIA mice from TP-induced toxicity through modulation of the cytochrome P450 and glutathione metabolism pathways. Frontiers Media S.A. 2022-06-23 /pmc/articles/PMC9260116/ /pubmed/35814255 http://dx.doi.org/10.3389/fphar.2022.908227 Text en Copyright © 2022 Yan, Yan, Zhang, Wang, Shi, Jiang, Zhao, Wu and Zeng. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Pharmacology
Yan, Min
Yan, Yinyin
Zhang, Zhenqiang
Wang, Guoqiang
Shi, Wenbo
Jiang, Mengyuan
Zhao, Junwei
Wu, Xiangxiang
Zeng, Huahui
The Effect of Triptolide Combined With Crocin on Arthritis in Mice: From Side Effect Attenuation to Therapy
title The Effect of Triptolide Combined With Crocin on Arthritis in Mice: From Side Effect Attenuation to Therapy
title_full The Effect of Triptolide Combined With Crocin on Arthritis in Mice: From Side Effect Attenuation to Therapy
title_fullStr The Effect of Triptolide Combined With Crocin on Arthritis in Mice: From Side Effect Attenuation to Therapy
title_full_unstemmed The Effect of Triptolide Combined With Crocin on Arthritis in Mice: From Side Effect Attenuation to Therapy
title_short The Effect of Triptolide Combined With Crocin on Arthritis in Mice: From Side Effect Attenuation to Therapy
title_sort effect of triptolide combined with crocin on arthritis in mice: from side effect attenuation to therapy
topic Pharmacology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9260116/
https://www.ncbi.nlm.nih.gov/pubmed/35814255
http://dx.doi.org/10.3389/fphar.2022.908227
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