Rapid and highly specific detection of site-specific 5-hydroxymethylcytosine based on peroxotungstate oxidation and mismatch ligation-based LAMP

We have developed a rapid and specific method for site-specific 5-hydroxymethylcytosine (5hmC) quantification at single-base resolution. This bisulfite-free method integrates the peroxotungstate oxidization with the mismatched probe-assisted ligation to guarantee the specificity. Moreover, the high-...

Descripción completa

Detalles Bibliográficos
Autores principales: Zhang, Zhenhao, He, Tong, Qi, Yan, Dai, Yuxuan, Lao, Kejing, Gou, Xingchun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2022
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9261916/
https://www.ncbi.nlm.nih.gov/pubmed/35865199
http://dx.doi.org/10.1039/d2ra03310k

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9261916/
https://www.ncbi.nlm.nih.gov/pubmed/35865199
http://dx.doi.org/10.1039/d2ra03310k

Ejemplares similares